Plasmid DNA damage induced by helium atmospheric pressure plasma jet

  • Xu Han
  • William A. Cantrell
  • Erika E. Escobar
  • Sylwia Ptasinska
Regular Article

DOI: 10.1140/epjd/e2014-40753-y

Cite this article as:
Han, X., Cantrell, W., Escobar, E. et al. Eur. Phys. J. D (2014) 68: 46. doi:10.1140/epjd/e2014-40753-y
Part of the following topical collections:
  1. Topical Issue: Nano-scale Insights into Ion-beam Cancer Therapy

Abstract

A helium atmospheric pressure plasma jet (APPJ) is applied to induce damage to aqueous plasmid DNA. The resulting fractions of the DNA conformers, which indicate intact molecules or DNA with single- or double-strand breaks, are determined using agarose gel electrophoresis. The DNA strand breaks increase with a decrease in the distance between the APPJ and DNA samples under two working conditions of the plasma source with different parameters of applied electric pulses. The damage level induced in the plasmid DNA is also enhanced with increased plasma irradiation time. The reactive species generated in the APPJ are characterized by optical emission spectra, and their roles in possible DNA damage processes occurring in an aqueous environment are also discussed.

Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Xu Han
    • 1
  • William A. Cantrell
    • 1
  • Erika E. Escobar
    • 1
  • Sylwia Ptasinska
    • 1
  1. 1.Radiation Laboratory and Department of Physics, University of Notre DameNotre DameUSA

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