Abstract
Molecular genetic analysis revealed a polymorphism for the Md-Exp7 gene that controls the biosynthesis of expansin in wild species of the genus Malus. Eight allelic forms of the gene were identified, four of which were unique. The regularities in the allele distribution in 37 genotypes were found to be in accordance with their systematic positions. The trait was found to be broadly diverse both among the sections of the genus and within a particular section. Variants of the gene were identified in representatives of one species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barsukova, O.N., Species, varieties, and forms of the genus Malus Mill.: immunological characterization, in Katalog mirovoi kollektsii VIR (Catalog of the VIR World Collection), St. Petersburg, 2007, vol. 781.
Costa, F., Van de Weg, W., and Stella, S., Map position and functional allelic diversity of Md-Exp7, a new putative expansin gene associated with fruit softening in apple (Malus × domestica Borkh.) and pear (Pyrus communis), Tree Genet. Genomes, 2008, vol. 4, pp. 575–586.
Jacquemin, J.-M. Baudoin, J.-P., et al., Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels, Biotechnol. Agron. Soc. Environ., 2006, vol. 10, no. 2, pp. 77–81.
Langenfel’d, V.T., Yablonya: Morfologicheskaya evolyutsiya, filogeniya, geografiya, sistematika (Apple Tree: Morphological Evolution, Phylogeny, Geography, and Systematics), Riga: Zinatie, 1991.
McQueen-Mason, S. and Cosgrove, D.J., Disruption of hydrogen bonding between wall polymers by proteins that induce plant wall extension, Proc. Natl. Acad. Sci. USA, 1994, vol. 91, pp. 6574–6578.
Nybom, H., Ahmadi-Afzadi, M., Sehic, J., and Maarten, H., DNA marker-assisted evaluation of fruit firmness at harvest and post-harvest fruit softening in a diverse apple germplasm, Tree Genet. Genomes, 2012, vol. 9, no. 1, pp. 279–290.
Ponomarenko, V.V. and Ponomarenko, K.V., Genofond vidov roda Malus Mill. Yablonya (The Gene Pool of Species of the Genus Malus Mill. Apple Tree), St. Petersburg: Obshchestvo pamyati igymenii Taisii, 2013.
Puchooa, D.A., A simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.), Afr. J. Biotechnol, 2004, vol. 3, no. 4, pp. 253–255.
Shamshin, I.N., Savel’ev, N.I., and Kudryavtsev, A.M., Allelic diversity of the MD-EXP 7U gene varieties of apple and pear, Vestn. Michurinsk. Gos. Agrar. Univ., 2012, part 1, no. 4, pp. 23–26.
Sharova, E.I., Expansins: proteins involved in cell wall softening during plant growth and morphogenesis, Russ. J. Plant Physiol., 2007, vol. 54, no. 6, pp. 713–727.
Urbanovich, O.Yu., Kuzmitskaya, P.V., Kozlovskaya, Z.A., et al., The allelic composition of MD-ACS1, MDACO1, and Md-Exp7 genes in apple (Malus × domestica Borkh.) cultivars with different shelf life of fruits, Vestn. Nats. Akad. Nauk Belarusi, Ser. Biol. Nauki, 2013, no. 3, pp. 47–55.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © N.I. Savel’ev, I.N. Shamshin, N.N. Savel’eva, A.S. Lyzhin, 2014, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2014, Vol. 18, No. 4/1, pp. 713–717.
Rights and permissions
About this article
Cite this article
Savel’ev, N.I., Shamshin, I.N., Savel’eva, N.N. et al. Polymorphism of the Md-Exp7 gene for the biosynthesis of expansin in wild species of the genus Malus Mill.. Russ J Genet Appl Res 5, 216–219 (2015). https://doi.org/10.1134/S2079059715030156
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S2079059715030156