Skip to main content
Log in

Application of the electrophoresis of cotyledon storage protein and ISSR-markers to the identification of hybrids between Sorbus sibirica Hedl. and Sorbocotoneaster pozdnjakovii Pojark

  • Published:
Russian Journal of Genetics: Applied Research

Abstract

It is shown that cotyledon protein SDS-electrophoresis and inter-simple sequence repeat DNA markers (ISSRs) can be used in identification of hybrid genotypes in Sorbus sibirica Hedl. and Sorbocotoneaster pozdnjakovii Pojark. Recombination of molecular and morphological characters was observed in hybrids, which proves close relationships of the parental genomes and, as a consequence, the potential of utilizing Sorbocotoneaster pozdnjakovii genotypes in breeding of Sorbus cultivars. The results confirm that the genus Sorbocotoneaster arose as a result of Sorbus and Cotoneaster hybridization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Agafonov, A.V. and Agafonova, O.V., SDS-PAGE of endosperm proteins in the members of the genus Elymus L. with different genomic structure, Sib. Biol. Zh., 1992, no. 3, pp. 7–12.

    Google Scholar 

  • Evans, R.C. and Campbell, C.S., The origin of the apple subfamily (Maloideae; Rosaceae) is clarified by DNA sequence data from duplicated GBSSI genes, Am. J. Bot., 2002, vol. 89, no. 9, pp. 1478–1484.

    Article  CAS  PubMed  Google Scholar 

  • Gladkova, V.N., Cytological study of spontaneous hybridogenic genus Sorbocotoneaster Pojark, Botan. Zh., 1967, vol. 52, no. 3, pp. 981–983.

    Google Scholar 

  • Koropachinskii, I.Yu. and Vstovskaya, T.N., Drevesnye rasteniya Aziatskoi Rossii (Woody Plants of Asian Russia), Novosibirsk: Geo, 2012.

    Google Scholar 

  • Krasnaya kniga Respubliki Sakha (Yakutii). Redkie i nakhodyashchiesya pod ugrozoi ischeznoveniya vidy rastenii i gribov (The Red Data Book of the Republic of Sakha (Yakutia). Rare and Endangered Species of Plants and Fungi), Yakutsk: NIPK Sakhapoligrafizdat, 2000, pp. 176–177.

  • Krasnaya kniga Rossiiskoi Federatsii (rasteniya i griby) (The Red Data Book of the Russian Federation (Plants and Fungi)), Moscow: Tovar. Nauch. Izd. KMK, 2008.

  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, no. 5259, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

  • Liljefors, A., Studies on propagation, embryology and pollination in Sorbus, Acta Horti Bergiani, 1953, vol. 16, pp. 277–329.

    Google Scholar 

  • Liljefors, A., Cytological studies in Sorbus, Acta Horti Bergiani, 1955, vol. 17, pp. 47–113.

    Google Scholar 

  • Nei, M. and Li, W.-H., Mathematical model for studying genetic variation in terms of restriction endonucleases, Proc. Natl. Acad. Sci. U.S.A., 1979, vol. 76, no. 10, pp. 5269–5273.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Osborn, T.B., Rastitel’nye belki (Plant Proteins), Moscow: Biomedgiz, 1935.

    Google Scholar 

  • Peer, Van and Wachter, R.D., TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment, Comput. Appl. Biosci., 1994, vol. 10, pp. 569–570.

    PubMed  Google Scholar 

  • Petrova, A.E., Nazarova, E.I., and Romanova, A.Yu., Introduction of Sorbocotoneaster pozdnjakovii, an endemic of South Yakutia, in Botanicheskie issledovaniya v kriolitozone (Botanical Studies in Permafrost), Yakutsk: YaNTs SO RAN, 1992, pp. 74–80.

    Google Scholar 

  • Programma i metodika otdalennoi gibridizatsii plodovykh i yagodnykh kul’tur (Program and Technique of Hybridization of Fruit and Berry Crops), Nesterov, Ya.S., Ed., Michurinsk: TSGL im. I.V. Michurina, 1972.

    Google Scholar 

  • Puchooa, D.A., Simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.), Afr. J. April, 2004, vol. 3, no. 4, pp. 253–255.

    CAS  Google Scholar 

  • Redkie i ischezayushchie rasteniya Sibiri (Rare and Endangered Plants of Siberia), Novosibirsk: Nauka, 1980.

  • Robertson, A., Newton, A.C., and Ennos, R.A., Breeding systems and continuing evolution in the endemic Sorbus taxa on Arran, Heredity, 2004, vol. 93, pp. 487–495.

    Article  CAS  PubMed  Google Scholar 

  • Shcherban’, A.B., Reorganization of plant genome during allopolyploidization, Vavilov Zh. Genet. Selekts., 2013, vol. 17, no. 2, pp. 277–285.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to S. V. Asbaganov.

Additional information

Original Russian Text © S.V. Asbaganov, E.V. Kobozeva, A.V. Agafonov, 2014, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2014, Vol. 18, No. 3, pp. 486–496.

The article was translated by the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Asbaganov, S.V., Kobozeva, E.V. & Agafonov, A.V. Application of the electrophoresis of cotyledon storage protein and ISSR-markers to the identification of hybrids between Sorbus sibirica Hedl. and Sorbocotoneaster pozdnjakovii Pojark. Russ J Genet Appl Res 5, 33–40 (2015). https://doi.org/10.1134/S2079059715010013

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S2079059715010013

Keywords

Navigation