Skip to main content
Log in

Wheat promoter sequences for transgene expression

  • Published:
Russian Journal of Genetics: Applied Research

Abstract

Genetic engineering techniques and transgenic plants are frequently used in plant molecular genetics. Promoters from wheat genes with verified patterns of activity (annotated in the TransGene Promoter Database (TGP)) are discussed. The TGP database can be used as an information resource for planning various genetic engineering experiments.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Altpeter, F., Varshney, A., Aderhalden, O., et al., Stable Expression of a Defense-Related Gene in Wheat Epidermis under Transcriptional Control of a Novel Promoter Confers Pathogen Resistance, Plant. Mol. Biol., 2005, vol. 57, pp. 271–283.

    Article  PubMed  CAS  Google Scholar 

  • Berna, A. and Bernier, F., Regulation by Biotic and Abiotic Stress of a Wheat Germ in Gene Encoding Oxalate Oxidase, a H2O2-Producing Enzyme, Plant Mol. Biol., 1999, vol. 39, pp. 539–549.

    Article  PubMed  CAS  Google Scholar 

  • Bilgin, M., Dedeoǧlu, D., Omirulleh, S., et al., Meristem, Cell Division and S Phase-Dependent Activity of Wheat Histone H4 Promoter in Transgenic Maize Plants, Plant Sci., 1999, vol. 143, pp. 35–44.

    Article  CAS  Google Scholar 

  • Boutrot, F., Meynard, D., Guiderdoni, E., et al., The Triticum aestivum Non-Specific Lipid Transfer Protein (TaLtp) Gene Family: Comparative Promoter Activity of Six Taltp Genes in Transgenic Rice, Planta, 2007, vol. 225, pp. 843–862.

    Article  PubMed  CAS  Google Scholar 

  • Chen, L., Tu, Z., Hussain, J., et al., Isolation and Heterologous Transformation Analysis of a Pollen-Specific Promoter from Wheat (Triticum aestivum L.), Mol. Biol. Rep., 2010, vol. 37, pp. 737–744.

    Article  PubMed  Google Scholar 

  • Colot, V., Robert, L.S., Kavahagh, T.A., et al., Localization of Sequences in Wheat Endosperm Protein Genes Which Confer Tissue-Specific Expression in Tobacco, EMBO J., 1987, vol. 6, pp. 3559–3564.

    PubMed  CAS  Google Scholar 

  • Cong, L., Wang, C., Chen, L., et al., Expression of Phytoene Synthase 1 and Carotene Desaturase CrtI Genes Result in an Increase in the Total Carotenoids Content in Transgenic Elite Wheat (Triticum aestivum L.), J. Agric. Food Chem., 2009, vol. 57, pp. 8652–8660.

    Article  PubMed  CAS  Google Scholar 

  • Cui, X., Zhang, Y., Zhao, F., et al., Molecular Characterization and Expression Analysis of Phosphate Transporter Gene TaPT2-1 in Wheat (Triticum aestivum L.), Front. Agricult. China, 2011, vol. 5, pp. 274–283.

    Article  Google Scholar 

  • Egawa, C., Kobayashi, F., Ishibashi, M., et al., Differential Regulation of Transcript Accumulation and Alternative Splicing of ADREB2 Homolog under Abiotic Stress Conditions in Common Wheat, Genes Genet. Syst., 2006, vol. 81, pp. 77–91.

    Article  PubMed  CAS  Google Scholar 

  • Evrard, A., Meynard, D., Guiderdoni, E., et al., The Promoter of the Wheat Puroindoline-a Gene (PinA) Exhibits a More Complex Pattern of Activity Than That of the PinB Gene and Is Induced by Wounding and Pathogen Attack in Rice, Planta, 2007, vol. 225, pp. 287–300.

    Article  PubMed  CAS  Google Scholar 

  • Fejes, E., Pay, A., Kanevsky, I., et al., A 268 Bp Upstream Sequence Mediates the Circadian Clock-Regulated Transcription of the Wheat Cab-1 Gene in Transgenic Plants, Plant. Mol. Biol., 1990, vol. 15, pp. 921–932.

    Article  PubMed  CAS  Google Scholar 

  • Furtado, A. and Henry, R.J., The Wheat Em Promoter Drives Reporter Gene Expression in Embryo and Aleurone Tissue of Transgenic Barley and Rice, Plant Biotechnol. J., 2005, vol. 3, pp. 421–434.

    Article  PubMed  CAS  Google Scholar 

  • Harholt, J., Bach, I.C., Lind-Bouquis, S., et al., Generation of Transgenic Wheat (Triticum aestivum L.) Accumulating Heterologous Endo-Xylanase or Ferulic Acid Esterase in the Endosperm, Plant Biotechnol. J., 2010, vol. 8, pp. 351–362.

    Article  PubMed  CAS  Google Scholar 

  • Van Herpen, T.W., Riley, M., Sparks, C., et al., Detailed Analysis of the Expression of an alpha-Gliadin Promoter and the Deposition of alpha-Gliadin Protein during Wheat Grain Development, Ann. Bot., 2008, vol. 102, pp. 331–342.

    Article  PubMed  Google Scholar 

  • Himmelbach, A., Zierold, U., Hensel, G., et al., A Set of Modular Binary Vectors for Transformation of Cereals, Plant Physiol., 2007, vol. 145, pp. 1192–1200.

    Article  PubMed  CAS  Google Scholar 

  • Huh, G.H., Nakayama, T., Meshi, T., and Iwabuchi, M., Structural Characteristics of Two Wheat Histone H2A Genes Encoding Distinct Types of Variants and Functional Differences in Their Promoter Activity, Plant. Mol. Biol., 1997, vol. 33, pp. 791–802.

    Article  PubMed  CAS  Google Scholar 

  • Ito, T., Fujimoto, Y., Nakayama, T., and Iwabuchi, M., A Farup-Stream Sequence of the Wheat Histone H3 Promoter Functions Differently in Rice and Tobacco Cultured Cells, Plant Cell Physiol., 1995, vol. 36, pp. 1281–1289.

    PubMed  CAS  Google Scholar 

  • Kobayashi, F., Ishibashi, M., and Takumi, S., Transcriptional Activation of Cor/Lea Genes and Increase in Abiotic Stress Tolerance through Expression of a Wheat Dreb2 Homolog in Transgenic Tobacco, Transgenic Res., 2008, vol. 17, pp. 755–767.

    Article  PubMed  CAS  Google Scholar 

  • Kovalchik, N., Li, M., Wittek, F., et al., Defensin Promoters as Potential Tools for Engineering Disease Resistance in Cereal Grains, Plant Biotechnol. J., 2010, vol. 8, pp. 47–64.

    Article  Google Scholar 

  • Kovalchuk, N., Smith, J., Pallotta, M., et al., Characterization of the Wheat Endosperm Transfer Cell-Specific Protein TaPR60, Plant. Mol. Biol., 2009, vol. 71, pp. 81–98.

    Article  PubMed  CAS  Google Scholar 

  • Lamacchia, C., Shewry, P.R., Di Fonzo, N., et al., Endosperm-Specific Activity of a Storage Protein Gene Promoter in Transgenic Wheat Seed, J. Exp. Bot., 2001, vol. 52, pp. 243–250.

    Article  PubMed  CAS  Google Scholar 

  • Lloyd, J.C., Raines, C.A., John, U.P., and Dyer, T.A., The Chloroplast FBPase Gene of Wheat: Structure and Expression of the Promoter in Photosynthetic and Meristematic Cells of Transgenic Tobacco Plants, Mol. Gen. Genet., 1991, vol. 225, pp. 209–216.

    Article  PubMed  CAS  Google Scholar 

  • Marcotte, W.R.J., Russell, S.H., and Quatrano, S.R., Abscisic Acid-Responsive Sequence from the Em Gene of Wheat, Plant Cell, 1989, vol. 1, pp. 969–976.

    PubMed  CAS  Google Scholar 

  • Miao, J., Sun, J., Liu, D., et al., Characterization of the Promoter of Phosphate Transporter TaPHT1.2 Differentially Expressed in Wheat Varieties, J. Genet. Genomics, 2009, vol. 36, pp. 455–466.

    Article  PubMed  CAS  Google Scholar 

  • Nagy, F., Boutry, M., Hsu, M.Y., et al., The 5’-Proximal Region of the Wheat Cab-1 Gene Contains a 268-bp Enhancer-Like Sequence for Phytochrome Response, EMBO J., 1987, vol. 6, pp. 2537–2542.

    PubMed  CAS  Google Scholar 

  • Oszvald, M., Gardonyi, M., Tamas, C., et al., Development and Characterization of a Chimaeric Tissue-Specific Promoter in Wheat and Rice Endosperm, In Vitro Cell. Dev. Biol. Plant, 2008a, vol. 44, pp. 1–7.

    CAS  Google Scholar 

  • Oszvald, M., Kang, T.J., Tomoskozi, S., et al., Expression of Cholera Toxin B Subunit in Transgenic Rice Endosperm, Mol. Biotechnol., 2008b, vol. 40, pp. 261–268.

    Article  PubMed  CAS  Google Scholar 

  • Ouellet, F., Vazquez-Tello, A., and Sarhan, F., The Wheat Wes120 Promoter Is Cold-Inducible in Both Monocotyledonous and Dicotyledonous Species, FEBS Lett., 1998, vol. 423, pp. 324–328.

    Article  PubMed  CAS  Google Scholar 

  • Peleg, G., Malter, D., and Wolf, S., Viral Infection Enables Phloem Loading of Gfp and Long-Distance Trafficking of the Protein, Plant J., 2007, vol. 51, pp. 165–172.

    Article  PubMed  CAS  Google Scholar 

  • Piston, F., Marin, S., Hernando, A., and Barro, F., Analysis of the Activity of a Gamma-Gliadin Promoter in Transgenic Wheat and Characterization of Gliadin Synthesis in Wheat by MALDI-TOF during Grain Development, Mol. Breed., 2009, vol. 23, pp. 655–667.

    Article  CAS  Google Scholar 

  • Ryan, P.R., Raman, H., Gupta, S., et al., The Multiple Origins of Aluminium Resistance in Hexaploid Wheat Include Aegilops tauschii and More Recent cis Mutations to TaALMT1, Plant J., 2010, vol. 64, pp. 446–455.

    Article  PubMed  CAS  Google Scholar 

  • Schuenmann, P.H.D., Coia, G., and Waterhouse, P.M., Bipharming the SimpliRED HIV Diagnostic Reagent in Barley, Potato and Tobacco, Mol. Breed., 2002, vol. 9, pp. 113–121.

    Article  CAS  Google Scholar 

  • Schweizer, P., Tissue-Specific Expression of a Defense-Related Peroxidase in Transgenic Wheat Potentiates Cell Death in Pathogen-Attacked Leaf Epidermis, Mol. Plant Pathol., 2008, vol. 9, pp. 45–57.

    Article  PubMed  CAS  Google Scholar 

  • Smirnova, O.G., Ibragimova, S.S., and Kochetov, A.V., Simple Database to Select Promoters for Plant Transgenesis, Transgenic Res., 2011. Doi: 10.1007/S11248011-9538-2.

  • Stoger, E., Williams, S., Keen, D., and Christou, P., Constitutive Versus Seed Specific Expression in Transgenic Wheat: Temporal and Spatial Control, Transgenic Res., 1999, vol. 8, pp. 73–82.

    Article  CAS  Google Scholar 

  • Stoger, E., Parker, M., Christou, P., and Casey, R., Pea Legumin Overexpressed in Wheat Endosperm Assembles into an Ordered Paracrystalline Matrix, Plant Physiol., 2001, vol. 125, pp. 1732–1742.

    Article  PubMed  CAS  Google Scholar 

  • Takumi, S., Koike, A., Nakata, M., et al., Cold-Specific and Light-Stimulated Expression of a Wheat (Triticum aestivum L.) Core Gene Wcor15 Encoding a Chloroplast-Targeted Protein, J. Exp. Bot., 2003, vol. 54, pp. 2265–2274.

    Article  PubMed  CAS  Google Scholar 

  • Taoka, K., Ohtsubo, N., Fujimoto, Y., et al., The Modular Structure and Function of the Wheat H1 Promoter with S Phase-Specific Activity, Plant Cell Physiol., 1998, vol. 39, pp. 294–306.

    Article  PubMed  CAS  Google Scholar 

  • Terada, R., Nakayama, T., Iwabuchi, M., and Shimamoto, K., A Wheat Histone H3 Promoter Confers Cell Division-Dependent and -Independent Expression of the GusA Gene in Transgenic Rice Plants, Plant J., 1993, vol. 3, pp. 241–252.

    Article  PubMed  CAS  Google Scholar 

  • Tittarelli, A., Milla, L., Vargas, F., et al., Isolation and Comparative Analysis of the Wheat RaPT2 Promoter: Identification in Silico of New Putative Regulatory Motifs Conserved between Monocots and Dicots, J. Exp. Bot., 2007, vol. 58, pp. 2573–2582.

    Article  PubMed  CAS  Google Scholar 

  • Wang, H.W., Kwon, H.J., Yim, W.C., et al., Expressional Diversity of Wheat NsLTP Genes: Evidence of Subfunctionalization Via Cis-Regulatory Divergence, Genetics, 2010, vol. 138, pp. 843–852.

    CAS  Google Scholar 

  • Wiley, P.R., Tosi, P., Evrard, A., et al., Promoter Analysis and Immunolocalization Show That Puroindolien Genes Are Exclusively Expressed in Starchy Endosperm Cells of Wheat Grain, Plant. Mol. Biol., 2007, vol. 64, pp. 125–136.

    Article  PubMed  CAS  Google Scholar 

  • Xu, Z.S., Ni, Z.Y., Liu, L., et al., Characterization of the TaAIDFa Gene Encoding a CRT/DRE-Binding Factor Responsive to Drought, High-Salt, and Cold Stress in Wheat, Mol. Genet. Genomics, 2008, vol. 280, pp. 497–508.

    Article  PubMed  CAS  Google Scholar 

  • Yang, P., Taoka, K., Nakayama, T., and Iwabuchi, M., Structural and Functional Characterization of Two Wheat Histone H2B Promoters, Plant. Mol. Biol., 1995, vol. 28, pp. 155–172.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, J., Liu, W., Yang, X., et al., Isolation and Characterization of Two Putative Cytokinin Oxidase Genes Related to Grain Number per Spike Phenotype in Wheat, Mol. Biol. Rep., 2011, vol. 38, pp. 2337–2347.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to O. G. Smirnova.

Additional information

Original Russian Text © O.G. Smirnova, A.V. Kochetov, 2012, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2012, Vol. 16, No. 1, pp. 224–231.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Smirnova, O.G., Kochetov, A.V. Wheat promoter sequences for transgene expression. Russ J Genet Appl Res 2, 434–439 (2012). https://doi.org/10.1134/S2079059712060123

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S2079059712060123

Keywords

Navigation