Abstract
The relationship between the morphological variability of the productivity characteristics and the genetic variability of the electrophoretic spectra of seed polypeptides in the valuable medicinal species Hedysarum theinum Krasnob was established based on analyzing six cenopopulations. The protocols for the microclonal propagation of advanced samples were developed. The most efficient multiplication was achieved in an MS medium supplemented with 5 μM BAP, 200 mg/L−1 glutathione, and 200 mg/L−1 casein hydrolyzate. The shoots were successfully rooted on the half-strength MS basal medium supplemented with 7 μM NAA.
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References
Agafonov, A.V. and Agafonova, O.V., Electrophoretic spectra of prolamin in the samples of different-origin Elymus trachycaulon, Genetika, 1990, vol. 26, no. 11, pp. 1992–2001.
Agafonova, O.V. and Agafonov, A.V., Povyshenie razreshayushchei sposobnosti elektroforeticheskogo metoda dlya taksonomicheskikh i genetiko-selektsionnykh issledovanii mnogoletnikh zlakov triby Pshenitsevykh (Triticeae) (Improvement of Resolution of Electrophoresis for Taxonomic and Genetic-Selection Studies of Permanent Grasses of Tribe Triticeae), Available from VINITI, 1991, Kiev, no. 2467-B91.
Arcioni, S., Mariotti, D., and Pezotti, M., Hedysarum coronarium L. in vitro conditions for plant regeneration from protoplasts and callus of various explants, J. Plant Physiol., 1985, vol. 121, no. 2, pp. 141–148.
Ayala, G.J., Maurao, C.A., Perez-Salas, S., et al., Enzyme variability in the Drosophilae Williston group. I. Genetic differentiation among species, Proc. Natl. Acad. Sci. U.S.A., 1970, no. 67, pp. 225–232.
Butenko, R.G., Kul’tura izolirovannykh tkanei i fiziologiya morfogeneza u rastenii (Tissue Cultures in Vitro and Physiology of Plant Morphogenesis), Moscow: Nauka, 1964, pp. 32–33.
Dong, Y., Tang, D., Zhang, N., Li, Y., Zhang, Ch., Li, L., and Li, M., Phytochemicals and biological studies of plants in genus Hedysarum, Chem. Cent. J., 2013, vol. 7, pp. 124–137.
Dorogina (Agafonova), O.V. and Agafonova, M.A., Identification of closely related species Hedysarum theinum, H. neglectum, and H. austrosibiricum (Fabaceae) by seed storage globulins, Bot. Zh., 2004, vol. 89, no. 10, pp. 1637–1645.
Enquist, B.J. and Niklas, K.J., Global allocation rules for patterns of biomass partitioning in seed plants, Nature, 2002, vol. 395, pp. 163–165.
Flora Sibiri (Siberian Flora), Polozhii, A.V. and Malysheva, L.I., Eds., Novosibirsk, 1994, vol. 9.
Harborne, J.B. and Williams, C.A., Advances in flavonoid research since 1992, Phytochemistry, 2000, vol. 55, pp. 481–504.
Kalinin, F.L., Sarnatskaya, V.V., and Polishchuk, V.E., Metody kul’tury tkanei v fiziologii i biokhimii rastenii (Methods of Tissue Cultures in Vitro Used in Plant Physiology and Biochemistry), Kiev: Naukova Dumka, 1980.
Karnaukhova, N.A., Bioecological diversity of Hedysarum from Khakassia flora and assessment of their position in natural and introduced conditions, Sib. Ekol. Zh., 1994, vol. 1, no. 6, pp. 581–587.
Kukushkina, T.A., Vysochina G.I., Karnaukhova, N.A., and Selyutina, I.Yu., Content of mangiferin and sum of xanthones in the plants of some wild-growing and introduced Hedysarum L. species, Rast. Resur., 2011, no. 1, pp. 99–106.
Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, no. 5259. pp. 680–685.
Lyapkova, N.S., Khadeeva, N.V., Shain, S.S., and Maisuryan, A.N., Development of tissue cultivation of Hedysarum L. in vitro, Biotekhnologiya, 1999, no. 1, pp. 55–61.
Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant., 1962, vol. 15, no. 2, pp. 473–497.
Nechepurenko, I.V., Polovinka, N.P., Sal’nikova, O.I., Pokrovskii, L.M., Komarova, N.I., Salakhutdinov, N.F., and Nechepurenko, S.B., Components of the ethylacetate extract of Hedysarum theinum Krasnob. roots, Chem. Nat. Compd., 2007, vol. 43, no. 1, pp. 5–9.
Neretina, O.V., Gromova, A.S., Lutsky, V.I., and Semenov, A.A., Component composition of species of the genus Hedysarum (Fabaceae), Rast. Resur., 2004, vol. 40, no. 4, pp. 111–137.
Tsenopopulyatsii rastenii (osnovnye ponyatiya i struktura) (Plant Communities: General Terms and Structure)), Moscow: Nauka, 1976.
Vdovitchenko, M.Yu., Kuzovkina, I.N., Paetz, Ch., and Schneider, B., Formation of phenolic compounds in the roots of Hedysarum theinum cultured in vitro, Russ. J. Plant Physiol., 2007, vol. 54, no. 4, pp. 536–544.
Volodarskaya, S.B. and Agafonova, O.V., Productivity and concentration of oligomeric catechins of Hedysarum theinum Krasnob. in central and southwestern Altai, Rast. Resur., 2000, vol. 26, no. 4, pp. 47–52.
Volodarskaya, S.B., Vinokurova, E.Yu., and Shul’ts, E.E., Chemical study of Hedysarum theinum, Mater. Mezhd. konf. posvyashch. Pamyati V.G. Minaeva “Fizicheskie i biokhimicheskie aspekty izucheniya lekarstvennykh rastenii” (Proc. Int. Conf. in Memory of V.G. Minaeva “Physical and Biochemical Study of Medical Plants”), Novosibirsk, 1998, pp. 18–1
Vysochina, G.I., Kukushkina, T.A., Karnaukhova, N.A., and Selyutina, I.Yu., Flavonoids of wild and introduced plants of some species of the genus Hedysarum L., Khim. Interesakh Ust. Razvit., 2011, no. 4, pp. 365–371.
Zaitsev, G.N., Matematicheskaya statistika v eksperimental’noi botanike (Mathematical Statistics Implemented in Experimental Botany), Moscow: Nauka, 1984.
Zhou, L.G. and Wu, J.Y., Development and application of medicinal plant tissue cultures for production of drugs and herbal medicines in China, Nat. Prod. Rep., 2006, vol. 23, pp. 789–810.
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Original Russian Text © A.A. Erst, T.V. Zheleznichenko, T.I. Novikova, O.V. Dorogina, E.V. Banaev, 2014, published in Sibirskii Ekologicheskii Zhurnal, 2014, No. 1, pp. 87–92.
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Erst, A.A., Zheleznichenko, T.V., Novikova, T.I. et al. Ecological and geographic variability of Hedysarum theinum and features of its propagation in vitro. Contemp. Probl. Ecol. 7, 67–71 (2014). https://doi.org/10.1134/S1995425514010053
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DOI: https://doi.org/10.1134/S1995425514010053