Xenogenic bone matrix treated with supercritical carbon dioxide as a potential osteoplastic material
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- Zalepugin, D.Y., Zaitzev, V.V., Tilkunova, N.A. et al. Russ. J. Phys. Chem. B (2015) 9: 1005. doi:10.1134/S1990793115070131
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A multistage environmentally safe method for processing xenogenic bone matrix using suband supercritical media has been developed. This method provides fast matrix delipidization in a medium of supercritical carbon dioxide and deproteinization by treatment with a 3% hydrogen peroxide solution followed by washing with subcritical water for removal of polar substances. The final treatment with supercritical carbon dioxide serves to remove residual nonpolar components. A method to control the porosity of xenogenic bone matrix at different stages of its purification has been suggested. Preliminary data indicate that a cellular test system based on an allogenic osteoid cell line Th-1 could be used for evaluation of primary adhesion and proliferation of cells on the surface of purified bone matrix.