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Cytokine-mediated regulation of expression of Gfi1 and U2afll4 genes by activated T-cells with various differentiation status in vitro

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Abstract

The dose-dependent effects of cytokines (IL-2, IL-7, and IL-15), which have a common γ-chain, on mRNA expression of U2afll4 and GFi1 genes involved in regulation of alternative splicing of the Ptprc gene, have been investigated in vivo using T-lymphocyte cultures with different degrees of differentiation. IL-2, IL-7, and IL-15 caused a similar unidirectional inhibitory effect of various severity on restimulated CD45RO+ T-cells exposed to an antigen-independent activation; they caused a dose-dependent decrease of the U2af1l4 gene expression, and an increase of Gfi1 gene expression. This may suggest formation of active forms of the CD45 receptor, and also limitation of the formation of low-molecular short splice variants of the CD45RO receptor. Under conditions of antigen-independent stimulation of naive CD45RA+ - cells rIL-7 and IL-15 exhibited opposite effects on U2af1l4 and Gfi1 gene expression. The increase of IL-7 concentrations in the incubation medium of naive cells was accompanied by a decrease in expression of both genes. IL-15 IL-7 exhibited opposite effects. Cytokines possessing a common Γ-chain (IL-2, IL-7, and IL-15) prevented antigen-independent differentiation of naive T-cells, by preventing the formation of polyclonal “surrogate” cells. In general, the study of the molecular mechanisms of genetic control determining homeostatic processes of T-cells in response to exposure to antigenic or non-antigenic treatments may be important in the construction of a general model of self-maintenance and differentiation of immune cells.

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References

  1. Stamm, S., Human Mol. Genet., 2002, vol. 20, pp. 2409–2416.

    Article  Google Scholar 

  2. Ip, J.Y., Tong, A., Pan, Q., Topp, J.D., Blencowe, B.J., and Lynch, K.W., RNA, 2007, vol. 4, pp. 563–572.

    Article  Google Scholar 

  3. Rodrigues, R., Grosso, A.R., and Moita, L., PLoS One, 2013, vol. 4. doi 10.1371

  4. Tong, A., Nguyen, J., and Lynch K.W., J. Biol. Chem., 2005, vol. 280, no. 46, pp. 38297–38304.

    Article  CAS  Google Scholar 

  5. Wu, Z., Yates, A.L., Hoyne, G.F., and Goodnow, C.C., J. Immunol., 2010, vol. 1, pp. 231–223.

    Article  Google Scholar 

  6. Mustelin, T. and Tasken, K., Biochem. J., 2003, vol. 371, pp. 15–27.

    Article  CAS  Google Scholar 

  7. Saunders, A.E. and Johnson, P., Cell Signal., 2010, vol. 3, pp. 339–348.

    Article  Google Scholar 

  8. Evsyukova, I., Somarelli, A.J., Gregory, G.S., and Garcia-Blanco, M.A., RNA Biology, 2010, vol. 4, pp. 462–473.

    Article  Google Scholar 

  9. Heyd, F., ten Dam, G., and Möröy, T., Nat. Immunol., 2006, vol. 8, pp. 859–867.

    Article  Google Scholar 

  10. Litvinova, L.S., Mazunin, I.O., Gutsol, A.A., Sokhonevich, N.A., Khaziakhmatova, O.G., and Kofanova, K.A., Mol. Biol., 2013, vol. 47, pp. 474–476. doi 10.1134/S0026893313040092

    Article  Google Scholar 

  11. Lynch, W.K. and Weiss, A., Mol. Cell. Biol., 2000, vol. 20, pp. 70–80.

    Article  CAS  Google Scholar 

  12. Lantz, O., Grandjean, I., Matzinger, P., and Di Santo, J.P., Nat. Immunol., 2000, vol. 1, pp. 54–58.

    Article  CAS  Google Scholar 

  13. Schluns, K.S. and Lefrançois, L., Immunology, 2013, vol. 4, pp. 269–279.

    Google Scholar 

  14. Litvinova, L.S., Sokhonevich, N.A., Gutsol, A.A., and Kofanova, K.A., Cell and Tissue Biology, 2013, vol. 7, pp. 539–544.

    Article  Google Scholar 

  15. Yarilin, A.A., Immunologiya (Immunology), Moscow: Geotar-Media, 2010.

    Google Scholar 

  16. Gutsol, A.A., Sokhonevich, N.A., Seledtsov, V.I., and Litvinova, L.S., Byull. Exper. Biol. Med., 2013, vol. 155, pp. 468–470. doi 10.1007/s10517-013-2182-5

    Google Scholar 

  17. Benczik, M. and Gaffen, S.L., Immunol. Invest., 2004, vol. 2, pp. 109–142.

    Article  Google Scholar 

  18. Kovanen, P.E., Young, L., Al-Shami, A., Rovella, V., Pise-Masison, C.A., Radonovich, M.F., Powell, J., Fu, J., Brady, J.N., Munson, P.J., and Leonard, W.J., Int. Immunol., 2005, vol. 8, pp. 1009–1021.

    Article  Google Scholar 

  19. Ma, A., Koka, R., and Burkett, P., Annu. Rev. Immunol., 2006, vol. 24, pp. 657–679.

    Article  CAS  Google Scholar 

  20. Boyman, O., Purton, J.F., Surh, C.D., and Sprent, J., Curr. Opin. Immunol., 2007, vol. 3, pp. 320–326.

    Article  Google Scholar 

  21. Singh, M., Basu, S., Camell, C., Couturier, J., Nudelman, R.J., Medina, M.A., Rodgers, J.R., and Lewis, D.E., Eur. J. Immunol., 2008, vol. 6, pp. 1522–1532.

    Article  Google Scholar 

  22. Tanel, A., Fonseca, S.G., Yassine-Diab, B., Bordi, R., Zeidan, J., Shi, Y., Benne, C., and Sékaly, R.P., Expert Rev. Vaccines, 2009, vol. 3, pp. 299–312.

    Article  Google Scholar 

  23. Le Campion, A., Pommier, A., Delpoux, A., Stouvenel, L., Auffray, C., Martin, B., Lucas, B., J. Immunol., 2012, vol. 7, pp. 3339–3346.

    Article  Google Scholar 

  24. De Arras, L., and Alper, S., PLoS Genet., 2013, vol. 10. e1003855 doi: 10.1371/jornal.pgen.1003855

    Google Scholar 

  25. Seledtsov, V.I., Litvinova, L.S., Goncharov, A.G., Shupletsova, V.V., Seledtsov, D.V., Gutsol, A.A., and Seledtsova, I.A., Tsitokiny i Vospalenie, 2010, vol. 4, pp. 9–15.

    Google Scholar 

  26. Martinez, M.N. and Lynch, W.K., Immunol. Rev., 2013, vol. 1, pp. 216–236.

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Innovation Park, Kant Baltic Federal University, ul. Botkina 3, Kaliningrad, 236000, Russia

    K. A. Yurova, N. A. Sokhonevich, O. G. Khaziakhmatova & L. S. Litvinova

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  1. K. A. Yurova
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  2. N. A. Sokhonevich
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  3. O. G. Khaziakhmatova
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  4. L. S. Litvinova
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Correspondence to L. S. Litvinova.

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Original Russian Text © K.A. Yurova, N.A. Sokhonevich, O.G. Khaziakhmatova, L.S. Litvinova, 2015, published in Biomeditsinskaya Khimiya.

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Yurova, K.A., Sokhonevich, N.A., Khaziakhmatova, O.G. et al. Cytokine-mediated regulation of expression of Gfi1 and U2afll4 genes by activated T-cells with various differentiation status in vitro. Biochem. Moscow Suppl. Ser. B 9, 166–173 (2015). https://doi.org/10.1134/S1990750815020146

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  • DOI: https://doi.org/10.1134/S1990750815020146

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