Abstract
The morphometric characteristics of NCTC cell clone 929 were compared before and after coming into contact with type I collagen added to cultivation medium. The cells were plated onto plastic in the form of a colony. The day after seeding, the cultivation medium was replaced by the same medium complemented with 0.1% type I collagen. The cells were incubated in this medium for 30 min more and then washed out of collagen. Using time-lapse shooting, the cell image at the colony edge was recorded for 7 h. The area of the cells and the degrees of their spreading and polarization were measured. It is shown that the contact with collagen did not affect the cell area, decreased cell spreading, and sharply reduced the portion of polarized cells. These results probably show the sensitivity of NCTC cells to the presence of type I collagen in cultivation medium and suggest that collagen plays an active role in formation by cells of long filopodia.
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Original Russian Text © Yu.P. Petrov, Yu.A. Negulyaev, N.V. Tsupkina, 2014, published in Tsitologiya, 2014, Vol. 56, No. 8, pp. 591–598.
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Petrov, Y.P., Negulyaev, Y.A. & Tsupkina, N.V. The morphology of NCTC cell clone 929 after contact with type I collagen added to culture medium. Cell Tiss. Biol. 8, 478–487 (2014). https://doi.org/10.1134/S1990519X14060066
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DOI: https://doi.org/10.1134/S1990519X14060066