Abstract
The dependence of the growth characteristics and monolayer formation on the initial cell plating concentration were studied on a permanent CHO cell line. The cells were cultivated under standard conditions on plastic substrate. Initial plating concentrations were varied as 1000, 2000, 3000, 4000, 5000, and 6000 cells/cm2. It was shown that the cell growth can be formally described by a standard S-shaped dependence. However, a more detailed analysis revealed inconsistency of the experimental and expected data. Specifically, the cell growth termination produced by monolayer formation does not coincide with the time when the theoretical curves approach a plateau. It is concluded that cell proliferation and monolayer formation are independent processes (at least in CHO cells). Both processes may be considered as analogs of proliferation and morphogenesis in metazoa. In addition, it is shown that the cessation of cell division is induced by reduction in the cell size to some limiting dimension and increasing of the cell polarization rather than contact inhibition of proliferation after the monolayer formation.
Similar content being viewed by others
References
Abercrombie, M. and Ambrose, E.J., The Surface Properties of Cancer Cells: A Review, Cancer Res., 1962, vol. 22, pp. 525–548.
Abercrombie, M., Contact Inhibition and Malignancy, Nature, 1979, vol. 281, pp. 259–262.
Ackermann, W.W., Rabson, A., and Kurtz, H., Growth Characteristics of Poliomyelitis Virus in HeLa Cell Cultures; Lack of Parallelism in Cellular Injury and Virus Increase, J. Exp. Med., 1954, vol. 100, pp. 437–450.
Desai, M., Bühler, T., Weller, P.H., and Brown, M.R., Increasing Resistance of Planktonic and Biofilm Cultures of Burkholderiacepacia to Ciprofloxacin and Ceftazidime during Exponential Growth, J. Antimicrob. Chemother., 1998, vol. 42, pp. 153–160.
Folkman, J. and Moscona, A., Role of Cell Shape in Growth Control, Nature, 1978, vol. 273, pp. 345–349.
Fujikawa, H. and Morozumi, S., Modeling Surface Growth of Escherichia coli on Agar Plates, Appl. Environ. Microbiol., 2005, vol. 71, pp. 7920–7926.
Ilio, K.Y., Sensibar, J.A., and Lee, C., Effect of TGF-beta 1, TGF-alpha, and EGF on Cell Proliferation and Cell Death in Rat Ventral Prostatic Epithelial Cells in Culture, J. Androl., 1995, vol. 16, pp. 482–490.
Khaitlina, S.Yu., Polymerization of beta-Like Actin from Scallop Adductor Muscle, FEBS Lett., 1986, vol. 198, pp. 221–224.
Murakami, C. and Kaeberlein, M., Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells, J. Vis. Exp., 2009, vol. 6, p. 1156.
Na, R.S., Zhao, Q.J., da Jin, P., Su, X.H., Chen, X.W., Guan, W.J., and Ma, Y.H., Establishment and Biological Characteristics of Ujumqin Sheep Fibroblast Line, Cytotechnology, 2010, vol. 62, pp. 43–52.
Nelson, P.J. and Daniel, T.O., Emerging Targets: Molecular Mechanisms of Cell Contact-Mediated Growth Control, Kidney Int., 2002, vol. 61, pp. S99–S105.
Oohira, A., Matsui, F., Oki, T., and Nogami, H., Deficiency of Density-Dependent Regulation of Cell Growth in the Culture of Skin Fibroblasts from Patients with Mucolipidosis III, J. Cell Sci., 1987, vol. 87, pp. 249–257.
Sinclair, W.K. and Ross, D.W., Models of Growth in Mammalian Cells, Biophys. J., 1969, vol. 9, pp. 1056–1070.
Vasil’ev, Yu.M. and Gel’fand, I.M., Vzaimodeystvie normal’nykh i neoplasticheskikh kletok so sredoi (Interaction of Normal and Neoplastic Cells with Environment), Moscow: Nauka, 1981.
Wieser, R.J., Renauer, D., Schüfer, A., Heck, R., Engel, R., Schütz, S., and Oesch, F., Growth Control in Mammalian Cells by Cell-Cell Contacts, Env. Health Perspect., 1990, vol. 88, pp. 251–253.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © Yu.P. Petrov, N.V. Tsupkina, 2012, published in Tsitologiya, 2012, Vol. 54, No. 10, pp. 754–760.
Rights and permissions
About this article
Cite this article
Petrov, Y.P., Tsupkina, N.V. Growth characteristics of CHO cells in culture. Cell Tiss. Biol. 7, 72–78 (2013). https://doi.org/10.1134/S1990519X13010124
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1990519X13010124