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Heat shock induces simultaneous rearrangements of all known cytoskeletal filaments in normal interphase fibroblasts

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Abstract

Hyperthermia (heat shock (HS)) induces changes in morphology of nucleoli, cytoplasmic organelles, and cytoskeleton. Responses to hyperthermia are, as a rule, similar in all types of eukaryote cells. However, there is no information on the uniformity of the cytoskeleton heat shock response (CHSR) in different cell types. This has led to the conclusion that the eukaryote CHSR depends on the cell type. We studied CHSR only in one cell type-in normal embryonic mouse fibroblasts (NEMFs) and in normal embryonic rat fibroblasts (NERFs), as well as in normal postnatal rat fibroblasts (NPRFs), by using the method of fluorescence microscopy. Incubation of the cells at 43°C led to a rearrangement of cytoskeleton. Responses of cytoskeleton to HS in NEMF, NERF, and NPRF were similar. Heat shock resulted in disassembly of bundles of actin filaments (AFs), marked changes in microtubule (MT) morphology, and collapse of intermediate filaments (IFs) around the nucleus. Rearrangements of different cytoskeleton filament types occurred simultaneously and were seen as soon as after 2–4 min. After 30–120 min of incubation at 43°C, the cells were still capable of rebuilding the actin cytoskeleton after the temperature had returned to normal (37°C). We believe that the cytoskeleton rearrangement under the action of HS can be of vital importance in cell protection against temperature stress. Data are discussed on possible coupling of the CHSR process with rearrangement of the protein synthesizing system, which leads to initiation and/or stimulation of synthesis of HS proteins.

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Abbreviations

AF:

actin-containing filaments

ATA:

bovine serum albumin, Triton-X-100, sodium azide in PBS

CHSR:

cytoskeleton heat shock response

HS:

heat shock

hsp:

heat shock proteins

IF:

intermediate filaments

MT:

microtubules

NEMF:

normal embryonic mouse fibroblasts

NERF:

normal embryonic rat fibroblasts

NPRF:

normal postnatal rat fibroblasts

PBS:

phosphate salt buffer solution

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Authors and Affiliations

  1. Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia

    L. P. Gavrilova & I. I. Korpacheva

  2. Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow oblast, Russia

    S. G. Semushina

  3. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia

    V. A. Yashin

Authors
  1. L. P. Gavrilova
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  2. I. I. Korpacheva
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  3. S. G. Semushina
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  4. V. A. Yashin
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Correspondence to L. P. Gavrilova.

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Original Russian Text © L.P. Gavrilova, I.I. Korpacheva, S.G. Semushina, V.A. Yashin, 2012, published in Tsitologiya, 2012, Vol. 54, No. 11, pp. 837–846.

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Gavrilova, L.P., Korpacheva, I.I., Semushina, S.G. et al. Heat shock induces simultaneous rearrangements of all known cytoskeletal filaments in normal interphase fibroblasts. Cell Tiss. Biol. 7, 54–63 (2013). https://doi.org/10.1134/S1990519X13010045

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  • DOI: https://doi.org/10.1134/S1990519X13010045

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