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Genomic mapping of chromatin proteins by using Daminv modification of an FLP-dependent DamID approach

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

To identify interactions of chromatin proteins with the genome of the cell type of interest that is a part of heterologous tissues and organs of Drosophila, an FLP-dependent DamID approach was recently developed [4], which does not require sorting of cells or nuclei. Here, a modification of this approach, Daminv, is described. The modified approach was validated by generating the binding pattern of the LAM protein, a component of the inner membrane of the nuclear envelope, with the genome of glial cells of the Drosophila larval central brains.

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Authors and Affiliations

  1. Netherlands Cancer Institute, Amsterdam, 1066 CX, the Netherlands

    A. V. Pindyurin

  2. Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk, 630090, Russia

    A. V. Pindyurin

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  1. A. V. Pindyurin
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Correspondence to A. V. Pindyurin.

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Original Russian Text © A.V. Pindyurin, 2017, published in Doklady Akademii Nauk, 2017, Vol. 472, No. 2, pp. 222–225.

The article was translated by the author.

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Pindyurin, A.V. Genomic mapping of chromatin proteins by using Daminv modification of an FLP-dependent DamID approach. Dokl Biochem Biophys 472, 15–18 (2017). https://doi.org/10.1134/S1607672917010057

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  • DOI: https://doi.org/10.1134/S1607672917010057

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