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Molecular analysis of transcription start sites of separate copies of the suffix short retroelement in genome of Drosophila

  • Biochemistry, Biophysics and Molecular Biology
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Abstract

In order to study TSS in the suffix element, we used total RNA isolated from ovaries of Drosophila melanogaster. Using a 5′-RACE System (Invitrogen) and 454 sequencing, we found the full-length suffix sense transcripts. However, most 5′-RACE reads (>70%) correspond to 5′-truncated transcripts lacking the first 33–39 nucleotides. The data may indicate that these RNAs are products of the RNAi-related silencing mech-anism producing small RNAs that are larger than piRNAs. The full-length suffix transcripts could arise either from the F element or from active separate copies of suffix.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, str. Vavilov 32, Moscow, 119991, Russia

    O. S. Zenina, D. M. Fedoseeva, O. V. Kretova & N. A. Tchurikov

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  1. O. S. Zenina
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  2. D. M. Fedoseeva
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  3. O. V. Kretova
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  4. N. A. Tchurikov
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Additional information

Original Russian Text © O.S. Zenina, D.M. Fedoseeva, O.V. Kretova, N.A. Tchurikov, 2013, published in Doklady Akademii Nauk, 2013, Vol. 449, No. 6, pp. 715–718.

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Zenina, O.S., Fedoseeva, D.M., Kretova, O.V. et al. Molecular analysis of transcription start sites of separate copies of the suffix short retroelement in genome of Drosophila . Dokl Biochem Biophys 449, 112–115 (2013). https://doi.org/10.1134/S1607672913020063

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  • DOI: https://doi.org/10.1134/S1607672913020063

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