Abstract
Proteolytic activity, which is inhibited in the presence of o-phenanthroline, was found in M. morganii ZM. Unlike grimelysin, intracellular proteases of M. morganii ZM actively cleave skeletal muscle actin. Several proteolytic proteins of M. morganii ZM were identified in cells by zymography with gelatin. Individual metalloproteinase (35 kDa) was isolated from the M. morganii ZM cell lysates by ammonium sulphate fractionation and purified by hydrophobic chromatography.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BLASTP:
-
Basic Local Alignment Search Tool Protein
References
Häse, C.C. and Finkelstein, R.A., Microbiol. Rev., 1993, vol. 57, pp. 823–837.
Stohl, E.A., Dale, E.M., Criss, A.K., and Seifert, H.S., MBio, 2013, vol. 4, pii: e00399–13. doi: 10.1128/mBio.00399-13
Kwon, K., Hasseman, J., Latham, S., Grose, C., Do, Y., Fleischmann, R.D., Pieper, R., and Peterson, S.N., BMC Biochem., 2011, vol. 12. doi: 10.1186/14712091-12-17
Kothary, M.H., McCardell, B.A., Frazar, C.D., Deer, D., and Tall, B.D., Appl. Environ. Microbiol., 2007, vol. 73, pp. 4142–4151.
Kuan, C.S., Wong, M.T., Choi, S.B., Chang, C.C., Yee, Y.H., Wahab, H.A., Normi, Y.M., Too, W.C., and Few, L.L., Int. J. Mol. Sci., 2011, vol. 12, pp. 4441–4455.
Wong, M.T., Choi, S.B., Kuan, C.S., Chua, S.L., Chang, C.H., Normi, Y.M., Too, W.C., Wahab, H.A., and Few, L.L., Int. J. Mol. Sci., 2012, vol. 13, pp. 901–917.
Khaitlina, S.Yu., Collins, J.H., Kuznetsova, I.M., Pershina, V.P., Synakevich, I.G., Turoverov, K.K., and Usmanova, A.M., FEBS Lett., 1991, vol. 279, pp. 49–51.
Matveyev, V.V., Usmanova, A.M., Morozova, A.V., Collins, J.H., and Khaitlina, S.Yu., Biochim. Biophys. Acta, 1996, vol. 1296, pp. 55–62.
Bozhokina, E., Khaitlina, S., and Adam, T., Biochem. Biophys. Res. Commun., 2008, vol. 367, pp. 888–892.
Demidyuk, I.V., Kalashnikov, A.E., Gromova, T.Y., Gasanov, E.V., Safina, D.R., Zabolotskaya, M.V., Rudenskaya, G.N., and Kostrov, S.V., Protein. Expr. Purif., 2006, vol. 47, pp. 551–561.
Bozhokina, E.S., Tsaplina, O.A., Efremova, T.N., Kever, L.V., Demidyuk, I.V., Kostrov, S.V., Adam, T., Komissarchik, Y.Y., and Khaitlina, S.Y., Cell. Biol. Int., 2011, vol. 35, pp. 111–118.
Tsaplina, O., Efremova, T., Demidyuk, I., and Khaitlina, S., FEBS J., 2012, vol. 279, pp. 264–274.
O’Hara, C.M., Brenner, F.W., and Miller, J.M., Clin. Microbiol. Rev., 2000, vol. 13, pp. 534–546.
Chen, Y.T., Peng, H.L., Shia, W.C., Hsu, F.R., Ken, C.F., Tsao, Y.M., Chen, C.H., Liu, C.E., Hsieh, M.F., Chen, H.C., Tang, C.Y., and Ku, T.H., BMC Genomics, 2012, Suppl. 13, no. 7, p. S4. doi: 10.1186/1471-216413-S7-S4
Khaitlina, S.Y., Moraczewska, J., and Strzelecka-Golaszewska, H., Eur. J. Biochem., 1993, vol. 218, pp. 911–920.
Edgar, R.C., Nucleic Acid Res., 2004, vol. 32, pp. 1792–1797.
Sambrook, J.K., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Press, 1989.
Laemmli, U.K., Nature, 1970, vol. 227, pp. 680–685.
Oliver, G.W., Stettler-Stevenson, W.G., and Kleiner, D.E., Zymography, casein zymography and reverse zymography; activity proteases and their inhibitors, in Handbook of Proteolyitic Enzymes, San Diego: Acad. Press, 1999.
Bradford, M.M., Anal. Biochem., 1976, vol. 72, pp. 248–254.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © N.M. Zamaliutdinova, L.F. Minnullina, M.R. Sharipova, A.M. Mardanova, 2014, published in Bioorganicheskaya Khimiya, 2014, Vol. 40, No. 6, pp. 682–687.
Rights and permissions
About this article
Cite this article
Zamaliutdinova, N.M., Minnullina, L.F., Sharipova, M.R. et al. New metalloendopeptidase of Morganella morganii ZM. Russ J Bioorg Chem 40, 628–633 (2014). https://doi.org/10.1134/S1068162014060156
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1068162014060156