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Phenothiazine derivatives as enhancers of peroxidase-dependent chemiluminescence

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Abstract

Some N-alkyl phenothiazines with different ionic groups were studied as enhancers of chemiluminescence catalyzed by soybean peroxidase. It was shown that under experimental conditions, the compounds with positively charged groups do not exhibit enhancing ability, while the addition of phenothiazines with negatively charged groups to a substrate mixture significantly increased the chemiluminescence intensity. The relationship between the enhancing activity of phenothiazines and their capacity for enzymatic oxidation by hydrogen peroxide was found. The enhancers discovered new opportunities for increasing the sensitivity of determination of analytes by chemiluminescent enzyme immunoassay.

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Abbreviations

EIA:

enzyme immunoassay

ECR:

enhanced chemiluminescence reaction

HRP:

horseradish peroxidase

SBP:

soybean peroxidase

PTPS:

sodium 3-(phenothiazin-10-yl)propane-1-sulfonate

PTP:

sodium 3-(phenothiazin-10-yl)propionate

CPTP:

sodium 3-(2-chlorophenothiazin-10-yl)propionate

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Original Russian Text © M.M. Vdovenko, A.Kh. Vorob’ev, I.Yu. Sakharov, 2013, published in Bioorganicheskaya Khimiya, 2013, Vol. 39, No. 2, pp. 200–205.

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Vdovenko, M.M., Vorobiev, A.K. & Sakharov, I.Y. Phenothiazine derivatives as enhancers of peroxidase-dependent chemiluminescence. Russ J Bioorg Chem 39, 176–180 (2013). https://doi.org/10.1134/S1068162013020155

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  • DOI: https://doi.org/10.1134/S1068162013020155

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