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Radiochemical approach in studying competitive adsorption of human serum albumin and ionic surfactants at the water/p-xylene interface

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Abstract

The competitive adsorption and distribution in the bulk of the aqueous solution/p-xylene system was studied, using tritium labeling and the scintillation phase method, for human serum albumin (HSA) and ionic surfactants: dodecyltrimethylammonium bromide (DTAB) and sodium dodecyl sulfate (SDS). Tritiumlabeled HSA, DTAB, and SDS of high specific radioactivity were obtained by the tritium thermal activation method and were purified by chromatography. Physicochemical studies were performed with HSA-SDS and HSA-DTAB mixtures at a protein concentration of 0.06 g l−1 and varied surfactant concentrations from 0.3 to 8.0 μM. The distribution ratio and the adsorption isotherm were obtained for each component of the mixture by the scintillation phase method. The effect of ultralow concentrations of surfactants on the competitive adsorption in mixtures with HSA and on the bulk distribution of the substances in the two-phase system was found. The parameters of intermolecular interaction in the adsorption layer were calculated, and a mechanism of formation of the adsorption layer was suggested.

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References

  1. Wang, R. and Schlenoff, J.B., Macromolecules, 1998, vol. 31, pp. 494–500.

    Article  CAS  Google Scholar 

  2. Soboleva, O.A., Korobkov, V.I., Summ, B.D., et al., Kolloidn. Zh., 1998, vol. 60, no. 6, pp. 826–830.

    Google Scholar 

  3. Badun, G.A., Lukashina, E.V., Batuk, O.N., et al., Mater. Sci. Eng. C, 2003, vol. 23, pp. 797–802.

    Article  Google Scholar 

  4. Sengupta, T. and Damodaran, S., J. Colloid Interface Sci., 1998, vol. 206, pp. 407–415.

    Article  CAS  Google Scholar 

  5. Sengupta, T., Razumovsky, L., and Damodaran, S., Langmuir, 1999, vol. 15, pp. 6991–7001.

    Article  CAS  Google Scholar 

  6. Damodaran, S. and Sengupta, T., J. Agric. Food Chem., 2003, vol. 51, pp. 1658–1665.

    Article  CAS  Google Scholar 

  7. Graham, D.E., Chatergoon, L., and Phillips, M.C., J. Phys. E: Sci. Instrum., 1975, vol. 8, pp. 696–699.

    Article  CAS  Google Scholar 

  8. Alent’ev, A.Yu. and Filatov, E.S., Radiokhimiya, 1991, vol. 33, no. 6, pp. 80–87.

    Google Scholar 

  9. Badun, G.A., Chernysheva, M.G., Pozdnyakova, V.Yu., and Fedoseev, V.M., Radiokhimiya, 2005, vol. 47, no. 6, pp. 536–540.

    Google Scholar 

  10. Chernysheva, M.G. and Badun, G.A., Mendeleev Commun., 2011, vol. 21, pp. 99–100.

    Article  CAS  Google Scholar 

  11. Chernysheva, M.G. and Badun, G.A., Langmuir, 2011, vol. 27, pp. 2188–2194.

    Article  CAS  Google Scholar 

  12. Badun, G.A., Chernysheva, M.G., Tyasto, Z.A., and Fedoseev, V.M., Vestn. Mosk. Gos. Univ., Ser. 2: Khim., 2009, vol. 50, no. 5, pp. 355–362.

    CAS  Google Scholar 

  13. Chernysheva, M.G., Soboleva, O.A., and Badun, G.A., Mendeleev Commun., 2008, vol. 18, pp. 345–346.

    Article  CAS  Google Scholar 

  14. Chakraborty, T., Chakraborty, I., Moulik, S.P., and Ghosh, S., Langmuir, 2009, vol. 25, pp. 3062–3074.

    Article  CAS  Google Scholar 

  15. Mehta, S.K., Bhawna, Bhasin, K.K., and Kumar, A., J. Colloid Interface Sci., 2008, vol. 323, pp. 426–434.

    Article  CAS  Google Scholar 

  16. Lu, R.-Ch., Cao, A.-N., Lai, L.-H., and Xiao, J.-X., Colloids Surf. B, 2008, vol. 64, pp. 98–103.

    Article  CAS  Google Scholar 

  17. Mikhilovskaya, A.A., Noskov, B.A., Lin, S.-Y., et al., J. Phys. Chem. B, 2011, vol. 115, pp. 9971–9979.

    Article  Google Scholar 

  18. Mir, M.A., Gull, N., Khan, J.M., et al., J. Phys. Chem. B, 2010, vol. 114, pp. 3197–3204.

    Article  CAS  Google Scholar 

  19. Badun, G.A., Chernysheva, M.G., and Ksenofontov, A.L., Radiochim. Acta, 2012, vol. 100, no. 6, pp. 401–408.

    Article  CAS  Google Scholar 

  20. Chernysheva, M.G. and Badun, G.A., J. Radioanal. Nucl. Chem., 2010, vol. 286, pp. 835–840.

    Article  CAS  Google Scholar 

  21. Dawson, R.M.C., Elliott, D.C., Elliott, W.H., and Jones, K.M., Data for Biochemical Research, Oxford: Clarendon, 1986, 3rd ed.

    Google Scholar 

  22. Fainerman, V.B., Lucassen-Reynders, E.H., and Miller, R., Adv. Colloid Interface Sci., 2003, vol. 106, pp. 237–259.

    Article  CAS  Google Scholar 

  23. Fainerman, V.B., Lucassen-Reynders, E.H., and Miller, R., Colloids Surf. A, 1998, vol. 143, pp. 141–165.

    Article  CAS  Google Scholar 

  24. Fainerman, V.B., Miller, R., and Wüstneck, R., J. Colloid Interface Sci., 1996, vol. 183, no. 1, pp. 24–36.

    Article  Google Scholar 

  25. Soboleva, O.A. and Ivanov, R.A., Vestn. Mosk. Gos. Univ., Ser. 2: Khim., 2011, vol. 52, no. 5, pp. 390–393.

    CAS  Google Scholar 

  26. Kaszuba, M., McKnight, D., Connah, M.T., et al., J. Nanoparticle Res., 2008, vol. 10, pp. 823–829.

    Article  CAS  Google Scholar 

  27. Neiman, L.A., Smolyakov, V.S., and Shishkov, A.V., Itogi Nauki Tekh., Ser.: Obshch. Probl. Fiz.-Khim. Biol., 1985, vol. 2.

  28. Badun, G.A., Soboleva, O.A., and Chernysheva, M.G., Mendeleev Commun., 2007, vol. 17, no. 6, pp. 357–358.

    Article  CAS  Google Scholar 

  29. Anfinsen, C.B., Edsall, J.T., and Richards, F.M., Adv. Protein Chem., 1985, vol. 37.

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Authors and Affiliations

  1. Chemical Faculty, Lomonosov Moscow State University, Leninskie gory 1, building 3, Moscow, 119991, Russia

    M. G. Chernysheva, I. A. Razzhivina, O. A. Soboleva & G. A. Badun

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  1. M. G. Chernysheva
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  2. I. A. Razzhivina
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  3. O. A. Soboleva
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  4. G. A. Badun
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Correspondence to M. G. Chernysheva.

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Original Russian Text © M.G. Chernysheva, I.A. Razzhivina, O.A. Soboleva, G.A. Badun, 2013, published in Radiokhimiya, 2013, Vol. 55, No. 2, pp. 180–185.

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Chernysheva, M.G., Razzhivina, I.A., Soboleva, O.A. et al. Radiochemical approach in studying competitive adsorption of human serum albumin and ionic surfactants at the water/p-xylene interface. Radiochemistry 55, 229–235 (2013). https://doi.org/10.1134/S106636221302015X

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  • DOI: https://doi.org/10.1134/S106636221302015X

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