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The role of protein oxidative modification and the cellular redox status in realization of apoptosis of MCF-7 breast adenocarcinoma cells

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Abstract

The aim of this study was to establish the role of oxidative modification of proteins and redox status in the realization of apoptosis of MCF-7 breast adenocarcinoma cells during cultivation with the SH-group blocker N-ethylmaleimide (NEM) and the SH-group protector 1,4-dithioerythritol (DTE). The activation of apoptosis in MCF-7 breast adenocarcinoma cells was shown to be due to the irreversible modification of redox-sensitive protein molecules. The presence of DTE in the culture medium of cancer cells caused reversible glutathionylation of protein molecules and did not change the number of apoptotic MCF-7 cells.

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Authors and Affiliations

  1. Siberian State Medical University, Ministry of Health of the Russian Federation, Moskovskii trakt 2, Tomsk, 634050, Russia

    E. V. Shakhristova, E. A. Stepovaya, O. L. Nosareva, V. D. Yakushina, V. V. Ivanov & V. V. Novitsky

  2. Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russia

    N. V. Ryazantseva

Authors
  1. E. V. Shakhristova
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  2. E. A. Stepovaya
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  3. N. V. Ryazantseva
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  4. O. L. Nosareva
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  5. V. D. Yakushina
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  6. V. V. Ivanov
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  7. V. V. Novitsky
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Corresponding author

Correspondence to N. V. Ryazantseva.

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Original Russian Text © E.V. Shakhristova, E.A. Stepovaya, N.V. Ryazantseva, O.L. Nosareva, V.D. Yakushina, V.V. Ivanov, V.V. Novitsky, 2016, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2016, No. 5, pp. 453–458.

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Shakhristova, E.V., Stepovaya, E.A., Ryazantseva, N.V. et al. The role of protein oxidative modification and the cellular redox status in realization of apoptosis of MCF-7 breast adenocarcinoma cells. Biol Bull Russ Acad Sci 43, 385–389 (2016). https://doi.org/10.1134/S1062359016050095

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  • DOI: https://doi.org/10.1134/S1062359016050095

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