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TLC determination of piroxicam, tenoxicam, celecoxib and rofecoxib in biological material

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Abstract

A rapid, sensitive and accurate TLC method has been developed for estimation of various pharmaceutical agents, such as piroxicam, tenoxicam, celecoxib and rofecoxib in human whole blood and urine. TLC analysis was performed on silica gel F254 plates with ethyl acetate-toluene-butylamine (2 : 2 : 1, v/v/v) and chloroform-acetone-toluene (6 : 2.5 : 1, v/v/v) as mobile phases. Densitometric scanning was performed at 254, 301 and 370 nm. The method was validated in terms of linearity, accuracy, precision, sensitivity and selectivity. The response was a linear function of concentration in the range of 1.4–39.1 μg/mL (r = 0.99). Precision of the assay was in the range 0.5–1.8%, and average accuracy was close to 100%. Presented method can be applied to routine quantification studies of chosen drugs in biological material.

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Authors and Affiliations

  1. Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, College of Medicine, Jagiellonian University, 9 Medyczna Str., Kraków, 30-688, Poland

    M. Starek, J. Krzek & P. Rotkegel

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  1. M. Starek
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  2. J. Krzek
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  3. P. Rotkegel
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Correspondence to M. Starek.

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Starek, M., Krzek, J. & Rotkegel, P. TLC determination of piroxicam, tenoxicam, celecoxib and rofecoxib in biological material. J Anal Chem 70, 351–359 (2015). https://doi.org/10.1134/S106193481503020X

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  • DOI: https://doi.org/10.1134/S106193481503020X

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