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Chromatographic determination of sildenafil in blood plasma using spectrophotometric and mass-spectrometric detection

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Abstract

Methods for the determination of sildenafil in human blood plasma by reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) and mass-spectrometric (MS) detection have been developed. Sildenafil was isolated from blood plasma by solid-phase extraction on Sep-Pak Vac tC18 cartridges with the recovery 95 ± 4%. The limits of quantification of sildenafil were 20 and 5 ng/mL using HPLC-UV and HPLC-MS methods, respectively.

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Authors and Affiliations

  1. CSU “Analytical Spectrometry”, St. Petersburg State Polytechnical University, ul. Gzhatskaya 27, POB 27, St. Petersburg, 195220, Russia

    D. V. Yaroshenko, A. V. Grigoriev & A. A. Sidorova

  2. St. Petersburg State University, Universitetskii pr. 26, Petrodvorets, St. Petersburg, 198504, Russia

    L. A. Kartsova

Authors
  1. D. V. Yaroshenko
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  2. A. V. Grigoriev
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  3. A. A. Sidorova
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  4. L. A. Kartsova
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Additional information

Original Russian Text © D.V. Yaroshenko, A.V. Grigoriev, A.A. Sidorova, L.A. Kartsova, 2013, published in Zhurnal Analiticheskoi Khimii, 2013, Vol. 68, No. 9, pp. 886–894.

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Yaroshenko, D.V., Grigoriev, A.V., Sidorova, A.A. et al. Chromatographic determination of sildenafil in blood plasma using spectrophotometric and mass-spectrometric detection. J Anal Chem 68, 801–808 (2013). https://doi.org/10.1134/S1061934813090128

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  • DOI: https://doi.org/10.1134/S1061934813090128

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