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Rapid mixing stopped-flow small-angle X-ray scattering study of lipoplex formation at beamline ID02@ESRF

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Abstract

Rapid mixing stopped-flow experiments, coupled with synchrotron small-angle X-ray scattering (SAXS) on the subsecond timescale, produce a vast amount of experimental structural data. The purpose of this work is to present the key steps of the SAXS data treatment for the case of self-assembly and complexation of cationic lipid particles (vesicles or cubosomes) with plasmid DNA in the course of a rapid mixing stopped-flow process. This approach is significant for the structural analysis of rapidly forming soft-matter biomacro-molecular delivery systems.

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Authors and Affiliations

  1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, CZ-16206, Prague, Czech Republic

    Borislav Angelov

  2. CNRS UMR8612 Institut Galien Paris-Sud, Univ Paris Sud 11, F-92296, Chatenay-Malabry, France

    Angelina Angelova & Sylviane Lesieur

  3. Laboratory for Soft Matter Electron Microscopy, Bayreuth Institute of Macromolecular Research, University of Bayreuth, D-95440, Bayreuth, Germany

    Markus Drechsler

Authors
  1. Borislav Angelov
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  2. Angelina Angelova
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  3. Markus Drechsler
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  4. Sylviane Lesieur
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Correspondence to Borislav Angelov.

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Angelov, B., Angelova, A., Drechsler, M. et al. Rapid mixing stopped-flow small-angle X-ray scattering study of lipoplex formation at beamline ID02@ESRF. J. Surf. Investig. 9, 105–110 (2015). https://doi.org/10.1134/S1027451015010279

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  • DOI: https://doi.org/10.1134/S1027451015010279

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