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Regulatory genomics: Combined experimental and computational approaches

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Abstract

The review describes combined experimental and computational approaches to the investigation of the mechanisms of transcriptional regulation of eukaryotic genes and organization of transcription regulatory regions. These include (a) an analysis of the factors affecting the affinity of TBP (TATA-binding protein) for the TATA box; (b) research on the patterns of chromatin mark distributions and their role in the regulation of gene expression; (c) a study of 3D chromatin organization; (d) an estimation of the effects of polymorphisms on gene expression via high-resolution ChIP-seq and DNase-seq techniques. It was demonstrated that combined experimental and computational approaches are very important for the current understanding of transcription regulatory mechanisms and the structural and functional organization of the regulatory regions controlling transcription.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia

    E. V. Ignatieva, O. A. Podkolodnaya, Yu. L. Orlov, G. V. Vasiliev & N. A. Kolchanov

  2. Novosibirsk State University, Novosibirsk, 630090, Russia

    E. V. Ignatieva, Yu. L. Orlov & N. A. Kolchanov

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  1. E. V. Ignatieva
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  2. O. A. Podkolodnaya
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  3. Yu. L. Orlov
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  4. G. V. Vasiliev
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  5. N. A. Kolchanov
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Correspondence to E. V. Ignatieva.

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Original Russian Text © E.V. Ignatieva, O.A. Podkolodnaya, Yu.L. Orlov, G.V. Vasiliev, N.A. Kolchanov, 2015, published in Genetika, 2015, Vol. 51, No. 4, pp. 409–429.

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Ignatieva, E.V., Podkolodnaya, O.A., Orlov, Y.L. et al. Regulatory genomics: Combined experimental and computational approaches. Russ J Genet 51, 334–352 (2015). https://doi.org/10.1134/S1022795415040067

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