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Ameliorating imidacloprid induced oxidative stress by 24-epibrassinolide in Brassica juncea L.

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Abstract

Pesticide toxicity causes oxidative stress to plants by generating reactive oxygen species (ROS). The aim of the present study was to observe the role of 24-epibrassinolide (24-EBL) in protection of Brassica juncea L. plants from oxidative stress caused by imidacloprid (IMI) pesticide. Generation of ROS, activities of antioxidative enzymes and chlorophyll contents were estimated using spectrophotometer, whereas organic acid contents were determined using gas chromatography-mass spectrometry (GC-MS). Statistical analysis of data revealed that 24-EBL significantly decreased ROS contents, accompanied by enhanced levels of shoot biomass, chlorophyll contents, organic acid contents and the activities of antioxidative enzymes in B. juncea plants under IMI toxicity.

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Abbreviations

24-EBL:

24-epibrassinolide

APOX:

ascorbate peroxidase

BRs:

brassinosteroids

CAT:

catalase

DHAR:

dehydroascorbate reductase

GC-MS:

gas chromatographymass spectrometry

GPOX:

guaiacol peroxidase

IMI:

imidacloprid

MLR:

multiple linear regression

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Plant Stress Physiology Lab, Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab, 143005, India

    A. Sharma, V. Kumar, A. K. Thukral & R. Bhardwaj

  2. Department of Botany and Environmental Science, Sri Guru Granth Sahib World University, Fatehgarh Sahib, Punjab, 140407, India

    M. K. Kanwar

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  1. A. Sharma
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  2. V. Kumar
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  3. M. K. Kanwar
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  4. A. K. Thukral
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  5. R. Bhardwaj
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Correspondence to A. Sharma.

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Sharma, A., Kumar, V., Kanwar, M.K. et al. Ameliorating imidacloprid induced oxidative stress by 24-epibrassinolide in Brassica juncea L.. Russ J Plant Physiol 64, 509–517 (2017). https://doi.org/10.1134/S1021443717040124

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  • DOI: https://doi.org/10.1134/S1021443717040124

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