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Isotope composition of carbon and nitrogen in tissues and organs of Betula pendula

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Abstract

Ratios of 13С/12C and 15N/14N isotopes were identified in different parts and organs of drooping birch (Betula pendula Roth) in preforest-steppe and pine-birch forests of the Middle Urals by mass spectrometry. The data were analyzed and interpreted from the perspective of biochemical processes of carbon and nitrogen metabolism in the leaf, cambial tissue, trunk wood, branches, roots, and in the soil. The lighter isotopic composition of carbon is characteristic for the leaves, trunk cambium as well as fine (<2 mm) roots. The trunk wood is characterized by the basal trend for 13C enrichment. The heavier carbon isotopic composition inversely related to metabolic activity of organs and tissues, in addition, 13С/12C ratio corresponds to the nitrogen content in the organs and tissues, indicating the metabolic control of carbon fractionation in woody plants. The isotopic composition of nitrogen in the aboveground parts of the plant (leaves, trunk cambium, wood) and in the medium and fine roots was significantly depleted in 15N (δ15N varies from 0 to–3‰), while main roots (δ15N = 0.6 ‰) and soil (δ15N = 2.4–6.7‰) were more enriched. The ratio of stable isotopes of carbon and nitrogen is an integrating index of carbon and nitrogen metabolism in plants.

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Abbreviations

δ13C:

heavy carbon (13C) isotope discrimination

δ15N:

heavy nitrogen (15N) isotope discrimination

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia

    P. Yu. Voronin & Vl. V. Kuznetsov

  2. Yeltsin Ural Federal University, Ekaterinburg, 620002, Russia

    V. A. Mukhin

  3. Far East Geological Institute, Far East Branch, Russian Academy of Sciences, Vladivostok, 690022, Russia

    T. A. Velivetskaya & A. V. Ignat’ev

Authors
  1. P. Yu. Voronin
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  2. V. A. Mukhin
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  3. T. A. Velivetskaya
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  4. A. V. Ignat’ev
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  5. Vl. V. Kuznetsov
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Correspondence to P. Yu. Voronin.

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Original Russian Text © P.Yu. Voronin, V.A. Mukhin, T.A. Velivetskaya, A.V. Ignat’ev, Vl.V. Kuznetsov, 2017, published in Fiziologiya Rastenii, 2017, Vol. 64, No. 2, pp. 127–132.

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Voronin, P.Y., Mukhin, V.A., Velivetskaya, T.A. et al. Isotope composition of carbon and nitrogen in tissues and organs of Betula pendula . Russ J Plant Physiol 64, 184–189 (2017). https://doi.org/10.1134/S1021443717010174

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  • DOI: https://doi.org/10.1134/S1021443717010174

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