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Gene transcription analysis during interaction between potato and Ralstonia solanacearum

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Abstract

Bacterial wilt (BW) caused by Ralstonia solanacearum (Rs) is an important quarantine disease that spreads worldwide and infects hundreds of plant species. The BW defense response of potato is a complicated continuous process, which involves transcription of a battery of genes. The molecular mechanisms of potato-Rs interactions are poorly understood. In this study, we combined suppression subtractive hybridization and macroarray hybridization to identify genes that are differentially expressed during the incompatible interaction between Rs and potato. In total, 302 differentially expressed genes were identified and classified into 12 groups according to their putative biological functions. Of 302 genes, 81 were considered as Rs resistance-related genes based on the homology to genes of known function, and they have putative roles in pathogen recognition, signal transduction, transcription factor functioning, hypersensitive response, systemic acquired resistance, and cell rescue and protection. Additionally, 50 out of 302 genes had no match or low similarity in the NCBI databases, and they may represent novel genes. Of seven interesting genes analyzed via RNA gel blot and semi-quantitative RT-PCR, six were induced, one was suppressed, and all had different transcription patterns. The results demonstrate that the response of potato against Rs is rapid and involves the induction of numerous various genes. The genes identified in this study add to our knowledge of potato resistance to Rs.

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Abbreviations

BW:

bacterial wilt

HR:

hypersensitive response

JA:

jasmonic acid

PCPI:

potato cysteine proteinase inhibitors

QTL:

quantitative trait loci

Rs :

Ralstonia solanacearum

SAR:

systemic acquired resistance

SSH:

suppression subtractive hybridization

TF:

transcription factors

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Genetics of Solanaceous Crops, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 10081, China

    G. C. Li, L. P. Jin, X. W. Wang, K. Y. Xie, S. W. Huang & D. Y. Qu

  2. Bio-Techology Research Center, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    G. C. Li & Y. Yang

  3. Plant Research International BV, PO Box 16, 6700 AA, Wageningen, The Netherlands

    E. A. G. van der Vossen

Authors
  1. G. C. Li
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  2. L. P. Jin
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  3. X. W. Wang
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  4. K. Y. Xie
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  5. Y. Yang
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  6. E. A. G. van der Vossen
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  7. S. W. Huang
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  8. D. Y. Qu
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Corresponding author

Correspondence to D. Y. Qu.

Additional information

Published in Russian in Fiziologiya Rastenii, 2010, Vol. 57, No.5, pp. 732–743

This text was submitted by the authors in English.

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Li, G.C., Jin, L.P., Wang, X.W. et al. Gene transcription analysis during interaction between potato and Ralstonia solanacearum . Russ J Plant Physiol 57, 685–695 (2010). https://doi.org/10.1134/S1021443710050122

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  • DOI: https://doi.org/10.1134/S1021443710050122

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