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Recovery of bean plants from boron-induced oxidative damage by zinc supply

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Abstract

The effects of zinc on growth, boron uptake, lipid peroxidation, membrane permeability (MP), lypoxygenase (LOX) activity, proline and H2O2 accumulation, and the activities of major antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX)) in bean plants were investigated under greenhouse conditions. Treatments consisted of control, 20 mg/kg B, and 20 mg/kg B plus 20 mg/kg Zn. When the plants were grown with 20 mg/kg Zn, B toxicity was less severe. Zinc supplied to soil counteracted the deleterious effects of B on root and shoot growth. Excess B significantly increased and Zn treatment reduced B concentrations in shoot and root tissues. Applied Zn increased the Zn concentration in the roots and shoots. While the concentrations of H2O2 and proline were increased by B toxicity, their concentrations were decreased by Zn supply. Boron toxicity increased the MP, malondialdehyde content, and LOX activity in excised bean leaves. Applied Zn significantly ameliorated the membrane deterioration. Compared with control plants, the activity of SOD was increased while that of CAT was decreased and APX remained unchanged in B-stressed plants. However, application of Zn decreased the SOD and increased the CAT and APX activities under toxic B conditions. It is concluded that Zn supply alleviates B toxicity by preventing oxidative membrane damage.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

LOX:

lypoxygenase

MP:

membrane permeability

Pro:

proline

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Ankara University, 06110, Ankara, Turkey

    A. Gunes, A. Inal & E. G. Bagci

Authors
  1. A. Gunes
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  2. A. Inal
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  3. E. G. Bagci
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Correspondence to A. Gunes.

Additional information

Published in Russian in Fiziologiya Rastenii, 2009, Vol. 56, No. 4, pp. 555–562.

This text was submitted by the authors in English.

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Gunes, A., Inal, A. & Bagci, E.G. Recovery of bean plants from boron-induced oxidative damage by zinc supply. Russ J Plant Physiol 56, 503–509 (2009). https://doi.org/10.1134/S1021443709040098

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  • DOI: https://doi.org/10.1134/S1021443709040098

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