Skip to main content
SpringerLink
Log in

A new gene of a TrfA type replication initiator found in a caprolactam/salicylate degradation plasmid pBS270

  • Short Communications
  • Published:
Molecular Biology Aims and scope Submit manuscript

Abstract

A mini-replicon was obtained for the caprolactam/salicylate degradation plasmid pBS270 (105 kb) of incompatibility group P-7 from Pseudomonas bacteria, and its nucleotide sequence was determined. A new gene that encodes a TrfA-like replication initiator was found on this replicon. The level of homology between this replication initiator and known proteins of the TrfA family suggests that the obtained replicon can be classified as IncP-1-like. The pBS270mini was shown to be chimeric.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

CAP/SAL plasmid:

caprolactam and salicylate biodegradation plasmid

ORF:

open reading frame

GI:

genomic island

References

  1. Esikova T.Z., Grishchenkov V.G., Kulakov L.A., Morenkova M.A., Boronin A.M. 1990. Incompatibility groups of plasmids responsible for ɛ-caprolactam biodegradation in bacteria of the genus Pseudomonas. Mol. Genet. Mikrobiol. Virusol. 4, 25–28.

    Google Scholar 

  2. Sevastsyanovich Y.R., Krasowiak R., Bingle L.E.H., Haines A.S., Sokolov S.L., Kosheleva I.A., Leuchuk A.A., Titok M.A., Smalla K., Thomas C.M. 2008. Diversity of IncP-9 plasmids of Pseudomonas. Microbiology (UK). 154, 2929–2941.

    Article  CAS  Google Scholar 

  3. Shintani M., Yano H., Habe H., Omori T., Yamane H., Tsuda M., Nojiri H. 2006. Characterization of the replication, maintenance, and transfer features of the IncP-7 plasmid pCAR1, which carries genes involved in carbazole and dioxin degradation. Appl. Environ. Microbiol. 72, 3206–3216.

    Article  PubMed  CAS  Google Scholar 

  4. Sambrook J., Russel D.W. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor, NY: Cold Spring Harbor Lab. Press.

    Google Scholar 

  5. van de Peer Y., de Wachter R. 1994. TREECON for Windows: A software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput. Appl. Biosci. 10, 569–570.

    PubMed  Google Scholar 

  6. Volkova O.V., Kosheleva I.A., Boronin A.M. 2012. Structure of replication initiation region in Pseudomonas IncP-7 streptomycin resistance plasmid Rms148. Mol. Biol. (Moscow). 46, 542–547.

    Article  CAS  Google Scholar 

  7. Stenger D.C., Lee M.W. 2011. Phylogeny of replication initiator protein TrfA reveals a highly divergent clade of incompatibility group P1 plasmids. Appl. Environ. Microbiol. 77, 2522–2526.

    Article  PubMed  CAS  Google Scholar 

  8. Stenger D.C., Lee M.W., Rogers E.E., Chen J. 2010. Plasmids of Xylella fastidiosa mulberry-infecting strains share extensive sequence identity and gene complement with pVEIS01 from the earthworm symbiont Verminephrobacter eiseniae. Physiol. Mol. Plant Pathol. 74, 238–245.

    Article  CAS  Google Scholar 

  9. Lee M.W., Rogers E.E., Stenger D.C. 2010. Functional characterization of replication and stability factors of an incompatibility group P-1 plasmid from Xylella fastidiosa. Appl. Environ. Microbiol. 77, 7734–7740.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    O. V. Volkova, A. V. Panov, I. A. Kosheleva, T. Z. Esikova & A. M. Boronin

  2. Pushchino State Institute of Natural Sciences, Pushchino, Moscow Region, 142290, Russia

    I. A. Kosheleva & A. M. Boronin

Authors
  1. O. V. Volkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Panov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. A. Kosheleva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Z. Esikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. M. Boronin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. V. Volkova.

Additional information

Original Russian Text © O.V. Volkova, A.V. Panov, I.A. Kosheleva, T.Z. Esikova, A.M. Boronin, 2013, published in Molekulyarnaya Biologiya, 2013, Vol. 47, No. 2, pp. 356–360.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Volkova, O.V., Panov, A.V., Kosheleva, I.A. et al. A new gene of a TrfA type replication initiator found in a caprolactam/salicylate degradation plasmid pBS270. Mol Biol 47, 316–319 (2013). https://doi.org/10.1134/S0026893313020179

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026893313020179

Keywords

Search

Navigation