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(-)-Epigallocatechin gallate regulates expression of apoptotic genes and protects cultured human lens epithelial cells under hyperglycemia

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Abstract

(-)-Epigallocatechin gallate (EGCG), the most abundant component in green tea, has a potent anti-apoptotic activity. The purpose of this study was to investigate the protective effects of EGCG and their molecular mechanisms on high glucose-induced apoptosis of human lens epithelial cells (HLEB-3). HLEB-3 cells were exposed to various concentrations of glucose and EGCG. Cell death was assessed by MTT assay and flow cytometry using annexin V and propidium iodide. The expression of the Bcl-2 family, c-fos, c-myc and p53 was measured by real-time PCR. EGCG decreased the Bcl-2/Bax expression stimulated by a high glucose. Moreover, EGCG suppressed the high glucose-induced expression of c-fos, c-myc and p53. These findings suggest that EGCG protects HLEB-3 cells from high glucose-induced apoptosis by regulating the gene expression of the Bcl-2 family, c-fos, c-myc and p53. Thus, EGCG may have a potential protective effect against diabetic cataract formation.

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Authors and Affiliations

  1. Eye Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China

    Panpan Ye, Kana Lin, Zhaochun Li, Jian Liu, Ke Yao & Wen Xu

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  1. Panpan Ye
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  2. Kana Lin
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  3. Zhaochun Li
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  4. Jian Liu
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  5. Ke Yao
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  6. Wen Xu
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Correspondence to Wen Xu.

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Published in Russian in Molekulyarnaya Biologiya, 2013, Vol. 47, No. 2, pp. 251–257.

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Ye, P., Lin, K., Li, Z. et al. (-)-Epigallocatechin gallate regulates expression of apoptotic genes and protects cultured human lens epithelial cells under hyperglycemia. Mol Biol 47, 222–227 (2013). https://doi.org/10.1134/S0026893313020106

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  • DOI: https://doi.org/10.1134/S0026893313020106

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