Abstract
Unlike in vertebrates and RNA viruses, the molecular clock has not been estimated so far for DNA viruses. The extended conserved central region (102 kb) of the orthopoxvirus genome and the DNA polymerase gene (3 kb) were analyzed in viruses representing several genera of the family Poxviridae. Analysis was based on the known dating of the variola virus (VARV) transfer from Western Africa to South America and previous data on the phylogenetic relatedness of modern West African and South American isolates of VARV. The mutation accumulation rate was for the first time estimated for these DNA viruses at (0.9–1.2) × 10−6 substitutions per site per year. It was assumed that poxviruses diverged from an ancestor approximately 500,000 years ago to form the recent species and that the ancestor of the genus Orthopoxvirus emerged approximately 300,000 years ago and gave origin to the modern species approximately 14,000 years ago.
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Kumar S., Hedges S.B. 1998. A molecular timescale for vertebrate evolution. Nature. 392, 917–920.
Russell P. 1998. Genetics. 5th ed. Menlo Park, CA: Addison Wesley Longman Inc.
Jenkins G.M., Rambaut A., Pybus O.G., Holmes E.C. 2002. Rates of molecular evolution in RNA viruses: A quantitative phylogenetic analysis. J. Mol. Evol. 54, 152–161.
McGeoch D.J., Cook S. 1994. Molecular phylogeny of the alphaherpesvirinae subfamily and a proposed evolutionary timescale. J. Mol. Biol. 238, 9–22.
McGeoch D.J., Cook S., Dolan A., Jamieson F.E., Telford E.A.R. 1995. Molecular phylogeny and evolutionary timescale for the family of mammalian herpesviruses. J. Mol. Biol. 247, 443–458.
McGeoch D.J., Gatherer D. 2005. Integrating reptilian herpesviruses into the family Herpesviridae. J. Virol. 79, 725–731.
Herniou E.A., Olszewski J.A., O’Reilly D.R., Cory J.S. 2004. Ancient coevolution of baculoviruses and their insect hosts. J. Virol. 78, 3244–3251.
Shchelkunov S.N., Marennikova S.S., Moyer R.W. 2005. Orthopoxviruses Pathogenic for Humans. Berlin: Springer.
Mikheev M.V., Feshchenko M.V., Shchelkunov S.N. 2004. Phylogenetic analysis of the gene for the orthopoxvirus chemokin-binding protein. Mol. Genet. Mikrobiol. Virusol. 1, 29–36.
Shchelkunov S.N., Totmenin A.V., Babkin I.V., Safronov P.F., Ryazankina O.I., Petrov N.A., Gutorov V.V., Uvarova E.A., Mikheev M.V., Sisler J.R., Esposito J.J., Jahrling P.B., Moss B., Sandakhchiev L.S. 2001. Human monkeypox and smallpox viruses: Genomic comparison. FEBS Lett. 509, 66–70.
Babkina I.N., Babkin I.V., Marennikova S.S., Sandakhchiev L.S., Shchelkunov S.N. 2004. Comparative restriction enzyme analysis of the genome in variola virus strains from the Russian collection. Mol. Biol. 38, 429–436.
Babkina I.N., Babkin I.V., Li Yu., Ropp S., Klein R., Damon I., Esposito J., Sandakhchiev L.S., Shchelkunov S.N. 2004. Phylogenetic comparison of the genome in different variola virus strains. Dokl. Akad. Nauk. 398, 818–822.
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. 1997. The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876–4882.
Hall T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41, 95–98.
Felsenstein J. 1989. PHYLIP: Phylogeny Inference Package (Version 3.2). Cladistics. 5, 164–166.
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Original Russian Text © I.V. Babkin, S.N. Shchelkunov, 2006, published in Molekulyarnaya Biologiya, 2006, Vol. 40, No. 1, pp. 20–24.
Presented for publication by A.A. Mironov
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Babkin, I.V., Shchelkunov, S.N. Time scale of Poxvirus evolution. Mol Biol 40, 16–19 (2006). https://doi.org/10.1134/S0026893306010031
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DOI: https://doi.org/10.1134/S0026893306010031