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Structure of the archaeal community in the Black Sea photic zone

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Abstract

Qualitative and quantitative analysis of the structure of the archaeal community of the photic zone of the Black Sea water column was carried out. Real-time PCR revealed 2 × 104 archaeal cells/mL (4.2% of the total cell number) at a 15-m depth. The structure of archaeal communities in the subsurface water column was investigated using the sequencing by synthesis technology (Illumina/Solexa) of the 16S rRNA genes. The Marine Group II phylogenetic cluster belonging to the phylum Euryarchaeota was the most numerous archaeal group (1.2–1.7 × 104 cells/mL). The Marine Group I phylogenetic cluster (phylum Thaumarchaeota) was the second most numerous group (40% of the free-living archaea or 7.7 × 103 cells/mL). Sequences of the ‘Nitrosopumilus’ cluster were revealed among Marine Group I sequences due to high homology (over 90%). A group of archaea belonging to the Deep-sea Hydrothermal Vent Euryarchaeotic Group 6 (DHVEG-6) (phylum Euryarchaeota) was also detected. The 16S rRNA gene sequences belonging to this cluster were revealed only in the suspension fraction. High homology level (over 90%) suggested classification of most DHVEG-6 sequences within the ‘Parvarchaeum’ cluster. In spite of a noticeable methane peak detected at 15-m depth, no sequences of methanogens were found.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia

    A. Y. Merkel, V. A. Korneeva, I. Yu. Tarnovetskii, A. L. Bryukhanov, E. A. Taranov & N. V. Pimenov

  2. Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia

    V. A. Korneeva, I. Yu. Tarnovetskii & A. L. Bryukhanov

  3. Shirshov Institute of Oceanology, Southern Branch, Gelendzhik, Russia

    V. K. Chasovnikov

  4. Immanuel Kant Baltic Federal University, Kaliningrad, Russia

    S. V. Toshchakov

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  1. A. Y. Merkel
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  8. N. V. Pimenov
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Original Russian Text © A.Y. Merkel, V.A. Korneeva, I.Yu. Tarnovetskii, A.L. Bryukhanov, V.K. Chasovnikov, E.A. Taranov, S.V. Toshchakov, N.V. Pimenov, 2015, published in Mikrobiologiya, 2015, Vol. 84, No. 4, pp. 476–484.

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Merkel, A.Y., Korneeva, V.A., Tarnovetskii, I.Y. et al. Structure of the archaeal community in the Black Sea photic zone. Microbiology 84, 570–576 (2015). https://doi.org/10.1134/S0026261715040128

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