Experimental Articles

Microbiology

, Volume 79, Issue 5, pp 679-687

Taxonomic characterization of the microorganisms associated with the cultivable diatom Synedra acus from Lake Baikal

  • Yu. R. ZakharovaAffiliated withLimnological Institute, Siberian Branch, Russian Academy of Sciences Email author 
  • , R. V. Adel’shinAffiliated withLimnological Institute, Siberian Branch, Russian Academy of Sciences
  • , V. V. ParfenovaAffiliated withLimnological Institute, Siberian Branch, Russian Academy of Sciences
  • , Ye. D. BedoshviliAffiliated withLimnological Institute, Siberian Branch, Russian Academy of Sciences
  • , Ye. V. LikhoshwayAffiliated withLimnological Institute, Siberian Branch, Russian Academy of Sciences

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Abstract

Determination of the taxonomic position of microorganisms associated with diatoms was carried out using microscopic, microbiological, and phylogenetic methods. Examination of the cultures of the fresh-water diatom Synedra acus grown in Lake Baikal water by epifluorescence and electron microscopy revealed that bacterial cells colonized the mucilage surrounding the cells of living microalgae and their cell surfaces and penetrate into the frustules of dead diatoms. A total of 13 strains of heterotrophic bacteria were isolated in pure cultures and described. Based on the results of analysis of their morphological, physiological, and biochemical properties, as well as on data obtained by 16S rRNA gene analysis, the following strains were identified to the species level: Sphingomonas sp., Variovorax paradoxus, Pseudomonas fluorescens, Microbacterium trichothecenolyticum, Rhodococcus sp., Caulobacter vibrioides, and Brevundimonas vesicularis.

Key words

microorganisms diatoms Lake Baikal 16S rRNA epifluorescence scanning and transmission electron microscopy