Abstract
To investigate the effects of silver nitrate (AgNO3) on model lipid membranes, we studied multibilayer membranes based on L-α-dipalmitoylphosphatidylcholine (DPPC) and AgNO3 aqueous solutions in a wide concentration range (up to 30 wt %) using differential scanning calorimetry. It was shown that the presence of AgNO3 leads to both an increase in the main phase transition temperature (T m ) of DPPC multibilayers and the appearance of an additional phase transition peak (T’ m ), suggesting an increase in both the density and heterogeneity of the lipid bilayer. Since the effect of nitrate ions (NO −3 ) was of the opposite nature (bilayer fluidizing), the integral effect of AgNO3 can be explained solely by the effect of silver ions (Ag+). With increasing AgNO3 concentration, the tendency to opposite changes in T m and T’m peak intensity was observed, so that at approximately 26 wt % AgNO3 the initial peak (T m ) disappeared. In the range of therapeutic Ag+ concentrations (up to 2 wt %), no significant changes in the phase state of model membranes were observed. This can be one of the causes of the absence of a damaging effect of silver-containing drugs on the host cells against the background of a pronounced bactericidal effect.
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Abbreviations
- DPPC:
-
L-α-dipalmitoylphosphatidylcholine
- DMPC:
-
L-α-dimyristoylphosphatidylcholine
- DSC:
-
differential scanning calorimetry
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Original Russian Text © O.V. Vashchenko, Iu.L. Iermak, A.O. Krasnikova, L.N. Lisetski, 2015, published in Biofizika, 2015, Vol. 60, No. 2, pp. 307–315.
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Vashchenko, O.V., Iermak, I.L., Krasnikova, A.O. et al. The effects of silver nitrate on the phase state of model multibilayer membranes. BIOPHYSICS 60, 244–250 (2015). https://doi.org/10.1134/S0006350915020207
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DOI: https://doi.org/10.1134/S0006350915020207