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Method of receiving differential images of objects autofluorescence in the process of photobleaching

  • Complex Systems Biophysics
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Abstract

The microscope with a high sensitive video camera and laser illumination was used to study autofluorescence changes of different structures in the photobleached region with a different speed. The work with images using the ImageJ program is described in application how to receive differential images of objects autofluorescence in the process of photobleaching.

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References

  1. Yan Sun, Hong Yu, Dong Zheng, et al., Arch. Pathol. Lab. Med. 135(10), 1335 (2011).

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  2. A. A. Klimov and D. A. Klimov, Biophysics 57(5), 899 (2012).

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  3. http://imagej.nih.gov/ij.

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    A. A. Klimov & D. A. Klimov

  2. Stereonic International Inc., 62 Seminol Way, Short Hills, NJ, 07078, USA

    A. A. Klimov & D. A. Klimov

Authors
  1. A. A. Klimov
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  2. D. A. Klimov
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Correspondence to A. A. Klimov.

Additional information

Original Russian Text. A.A. Klimov, D.A. Klimov, 2012, published in Biofizika, 2012, Vol. 57, No. 5, pp. 891–898.

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Klimov, A.A., Klimov, D.A. Method of receiving differential images of objects autofluorescence in the process of photobleaching. BIOPHYSICS 57, 692–698 (2012). https://doi.org/10.1134/S0006350912050090

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  • DOI: https://doi.org/10.1134/S0006350912050090

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