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Influence of ceruloplasmin and lactoferrin on the chlorination activity of leukocyte myeloperoxidase assayed by chemiluminescence

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Abstract

We demonstrate that addition of H2O2 to a mixture of myeloperoxidase (MPO), chloride and luminol immediately evokes a short intense flash of chemiluminescence (CL). This flash is diminished in the absence of MPO or chloride, and in the complete system it is suppressed by an MPO inhibitor azide, hypochlorite scavengers taurine or methionine, or an MPO peroxidase-cycle substrate guaiacol. Hence, this CL is mostly due to the MPO halogenation function; a measure of this activity is provided by the integral CL. With three independent methods (CL, taurine chlorination, and peroxidase assay) it is shown that MPO activity is suppressed by ceruloplasmin (Cp). Lactoferrin has no effect either on MPO or on the MPO-Cp complex. It is also shown that peroxidase inhibition by Cp is the stronger the larger is the MPO substrate, which suggests steric hindrances to substrate binding in the MPO-Cp complex. Importantly, the conventional chlorination and peroxidase assays detect MPO inhibition by Cp only at a large excess of the latter, whereas the CL assay reveals it at stoichiometric ratios characteristic of the naturally occurring protein complexes.

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Abbreviations

ABTS:

sodium 2,2′-azinobis(3-ethylbenzotriazoline-6-sulfonate

CL:

chemiluminescence

Cp:

ceruloplasmin

Lf:

lactoferrin

MPO:

myeloperoxidase

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Correspondence to O. M. Panasenko.

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Original Russian Text © O.M. Panasenko, A.V. Chekanov, I.I. Vlasova, A.V. Sokolov, K.V. Ageeva, M.O. Pulina, O.S. Cherkalina, V.B. Vasil’ev, 2008, published in Biofizika, 2008, Vol. 53, No. 4, pp. 573–581.

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Panasenko, O.M., Chekanov, A.V., Vlasova, I.I. et al. Influence of ceruloplasmin and lactoferrin on the chlorination activity of leukocyte myeloperoxidase assayed by chemiluminescence. BIOPHYSICS 53, 268–272 (2008). https://doi.org/10.1134/S0006350908040052

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  • DOI: https://doi.org/10.1134/S0006350908040052

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