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Mechanism of the action of biogenic chloramines and hypochlorite on the initial aggregation of platelets

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Abstract

The antiaggregant effect of two reactive oxidants—N,N-dichlorotaurine (a biogenic chloramine) and sodium hypochlorite—on the initial ADP-induced aggregation of rabbit blood platelets was studied. Platelet aggregation in reconstituted platelet-rich plasma was measured nephelometrically; an increase in the intensity of small-angle light scattering served as an index of aggregation. Addition of chloramine at relatively small concentrations (no greater than 1 mM available chlorine) directly to the reconstituted platelet-rich plasma suppressed the initial aggregation (formation of small aggregates) several times more strongly than preincubation of native plasma with chloramine. This suggests that N,N-dichlorotaurine realizes its antiaggregant effect on the platelet-rich plasma by directly interacting with cells. The effects of the inhibition of platelet aggregation in two variants of addition of high concentrations of N,N-dichlorotaurine did not differ significantly. In this case, a large amount of residual unreacted chloramine remained in the plasma, which caused the suppression of platelet aggregation during subsequent reconstitution of the platelet-rich plasma. Similar data were obtained in studying the antiaggregant effect of hypochlorite. N,N-Dichlorotaurine and hypochlorite at concentrations of 0.2–0.3 and 0.15 mM, respectively, strongly inhibited the initial aggregation of isolated platelets (approximately 2·108 cells/ml) preliminarily activated for 1.5 min by addition of 0.1–0.5 μM ADP. However, the antiaggregants had a more profound suppressive effect on the aggregation of unstimulated platelets. The antiaggregant effects of N,N-dichlorotaurine and hypochlorite probably stem from the oxidative modification of the sulfur-containing groups in platelet plasma membrane.

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Abbreviations

PRP:

platelet-rich plasma

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Original Russian Text © M.A. Murina, E.L. Savel’eva, D.I. Roschupkin, 2006, published in Biofizika, 2006, Vol. 51, No. 2, pp. 299–305.

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Murina, M.A., Savel’eva, E.L. & Roshchupkin, D.I. Mechanism of the action of biogenic chloramines and hypochlorite on the initial aggregation of platelets. BIOPHYSICS 51, 258–263 (2006). https://doi.org/10.1134/S0006350906020163

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  • DOI: https://doi.org/10.1134/S0006350906020163

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