Abstract
The antiaggregant effect of two reactive oxidants—N,N-dichlorotaurine (a biogenic chloramine) and sodium hypochlorite—on the initial ADP-induced aggregation of rabbit blood platelets was studied. Platelet aggregation in reconstituted platelet-rich plasma was measured nephelometrically; an increase in the intensity of small-angle light scattering served as an index of aggregation. Addition of chloramine at relatively small concentrations (no greater than 1 mM available chlorine) directly to the reconstituted platelet-rich plasma suppressed the initial aggregation (formation of small aggregates) several times more strongly than preincubation of native plasma with chloramine. This suggests that N,N-dichlorotaurine realizes its antiaggregant effect on the platelet-rich plasma by directly interacting with cells. The effects of the inhibition of platelet aggregation in two variants of addition of high concentrations of N,N-dichlorotaurine did not differ significantly. In this case, a large amount of residual unreacted chloramine remained in the plasma, which caused the suppression of platelet aggregation during subsequent reconstitution of the platelet-rich plasma. Similar data were obtained in studying the antiaggregant effect of hypochlorite. N,N-Dichlorotaurine and hypochlorite at concentrations of 0.2–0.3 and 0.15 mM, respectively, strongly inhibited the initial aggregation of isolated platelets (approximately 2·108 cells/ml) preliminarily activated for 1.5 min by addition of 0.1–0.5 μM ADP. However, the antiaggregants had a more profound suppressive effect on the aggregation of unstimulated platelets. The antiaggregant effects of N,N-dichlorotaurine and hypochlorite probably stem from the oxidative modification of the sulfur-containing groups in platelet plasma membrane.
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Abbreviations
- PRP:
-
platelet-rich plasma
References
S. T. Test, M. V. Lampert, P. J. Ossanna, et al., J. Clin. Invest. 74, 1341–1349 (1984).
E. L. Thomas, P. M. Bozeman, M. M. Jefferson, and C. C. King, J. Biol. Chem. 270, 2906–2913 (1995).
D. I. Roshchupkin, M. A. Murina, N. V. Adnoral, et al., Fiziol. Chel. 24, 113–120 (1998) [Human Physiol. 24 (3), 358–365 (1998)].
M. A. Murina, D. I. Roshchupkin, N. N. Kravchenko, et al., Biofizika 42 1279–1285 (1997).
M. A. Murina, V. N. Kuznetsvov, and D. I. Roshchupkin, Byull. Eksp. Biol. Med. 102, 676–678 (1986).
I. Porto, A. M. Leone, L. Nanni, et al., Blood Coagul. Fibrinol. 16, 97–104 (2005).
V. I. Sergienko and Yu. B. Vasiliev, Electrochemical Methods of Detoxication for Medical Use (Harwood Acad. Publ., GmbH, 1989).
M. A. Murina, V. I. Sergienko, and D. I. Roshchupkin, Official Bulletin of RF Committee on Patents and Trade Marks: Inventions, No. 30, 62 (1993)
M. A. Murina, D. I. Roshchupkin, and V. I. Sergienko, Official Bulletin of RF Committee on Patents and Trade Marks: Inventions, No. 1, 240 (2001).
M. A. Murina, O. D. Fesenko, V. I. Sergienko, et al., Byull. Eksp. Biol. Med. 134(7), 44–47 (2002).
G. V. R. Born, Nature 194, 927–929 (1962).
D. I. Roshchupkin, V. V. Berzhitskaya, and A. Yu. Sokolov, Biofizika 43, 503–510 (1998).
W. A. Prutz, Arch. Biochem. Biophys. 332, 110–120 (1996).
M. A. Murina, V. I. Sergienko, and D. I. Roshchupkin, Byull. Eksp. Biol. Med. 108(12), 702–704 (1989).
D. I. Roshchupkin and A. Yu. Sokolov, Official Bulletin of RF Committee on Patents and Trade Marks: Inventions, No. 28, 225 (1996).
N. G. Ardlie, M. A. Packham, and J. F. Mustard, Br. J. Haematol. 19, 7–17 (1970).
M. M. Frojmovic, J. G. Milton, and A. Duchasel, J. Lab. Clin. Med. 101, 964–974 (1983).
M. A. Murina, D. I. Roshchupkin, E. L. Savel’eva, and M. V. Zvereva, Physicochemical Bases of Functioning of Proteins and Their Complexes (Voronezh. Gos. Univ., Voronezh, 1998), pp. 162–165 [in Russian].
Yu. M. Torchinskii, Sulfur in Proteins (Nauka, Moscow, 1977) [in Russian].
S. T. Silk, K. T. H. Wong, and A. J. Marcus, Biochemistry 20, 391–397 (1981).
A. B. Samal’ and E. R. Loiko, Biokhimiya (Moscow) 65, 276–283 (2000).
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Original Russian Text © M.A. Murina, E.L. Savel’eva, D.I. Roschupkin, 2006, published in Biofizika, 2006, Vol. 51, No. 2, pp. 299–305.
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Murina, M.A., Savel’eva, E.L. & Roshchupkin, D.I. Mechanism of the action of biogenic chloramines and hypochlorite on the initial aggregation of platelets. BIOPHYSICS 51, 258–263 (2006). https://doi.org/10.1134/S0006350906020163
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DOI: https://doi.org/10.1134/S0006350906020163