Biochemistry (Moscow)

, Volume 71, Issue 2, pp 173–177

Protein composition and native state of pigments of thylakoid membrane of wheat genotypes differently tolerant to water stress

Authors

  • I. M. Guseynova
    • Institute of BotanyNational Academy of Sciences
  • S. Y. Suleymanov
    • Institute of BotanyNational Academy of Sciences
  • J. A. Aliyev
    • Institute of BotanyNational Academy of Sciences
Article

DOI: 10.1134/S000629790602009X

Cite this article as:
Guseynova, I.M., Suleymanov, S.Y. & Aliyev, J.A. Biochemistry (Moscow) (2006) 71: 173. doi:10.1134/S000629790602009X

Abstract

Protein composition and native state of chlorophylls were analyzed in two wheat (Triticum durum L.) genotypes with different tolerance to drought, Barakatli-95 (drought-tolerant) and Garagylchyg-2 (drought-sensitive), during water deficit. It is shown that the plants subjected to water deficit appear to have a slight increase in α-and β-subunits of CF1ATP-synthase complex (57.5 and 55 kD, respectively) in Barakatli-95 and their lower content in Garagylchyg-2. Steady-state levels of the core antenna of PS II (CP47 and CP43) and light-harvesting Chl a/b-apoproteins (LHC) II in the 29.5–24 kD region remained more or less unchanged in both wheat genotypes. The synthesis of 36 kD protein and content of low-molecular-weight polypeptides (21.5, 16.5, and 14 kD) were noticeably increased in the tolerant genotype Barakatli-95. Drought caused significant changes in the carotenoid region of the spectrum (400–500 nm) in drought-sensitive genotype Garagylchyg-2 (especially in the content of pigments of the violaxanthin cycle). A shift of the main band from 740–742 to 738 nm is observed in the fluorescence spectra (77 K) of chloroplasts from both genotypes under water deficiency, and there is a stimulation of the ratio of fluorescence band intensity F687/F740.

Key words

wheatpolypeptidesfluorescencedroughtchloroplastadaptation

Copyright information

© Pleiades Publishing, Inc. 2006