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Biocatalyzed transformation of progesterone by Geobacillus gargensis DSM 15378

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Abstract

The formation of secosteroids, 9,10-seco-4-pregnene-3,9,20-trione and 9,10-seco-4-pregnene-20α-hydroxy-3,9-dione, catalyzed by thermophilic bacterium Geobacillus gargensis DSM 15378 from progesterone is being reported for the first time. In addition, seven other hydroxy/keto progesterone derivatives, 7-ketoprogesterone, 11-ketoprogesterone, 20α-hydroxyprogesterone, 6β-hydroxyprogesterone, 6α-hydroxyprogesterone, 5α-pregnane-3,6,20-trione and 3β-hydroxy-5α-pregnane-6,20-dione, were identified through their spectral data. The influence of SDS, as a surfactant, on progesterone biotransformation products was investigated.

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Authors and Affiliations

  1. Department of Biological Science, Faculty of Science, Kuwait University, Safat, Kuwait

    N. E. Al-Beloshei, H. A. Al-Awadhi, R. A. R. Al-Khalaf, S. Oommen & M. Afzal

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  1. N. E. Al-Beloshei
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  2. H. A. Al-Awadhi
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  3. R. A. R. Al-Khalaf
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  4. S. Oommen
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  5. M. Afzal
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Correspondence to M. Afzal.

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Al-Beloshei, N.E., Al-Awadhi, H.A., Al-Khalaf, R.A.R. et al. Biocatalyzed transformation of progesterone by Geobacillus gargensis DSM 15378. Appl Biochem Microbiol 51, 321–328 (2015). https://doi.org/10.1134/S0003683815030023

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  • DOI: https://doi.org/10.1134/S0003683815030023

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