Abstract
An analytical system for a quantitative assessment of contributions of pre- and pro-components of the yeast α-factor leader to key stages of somatropin secretion in Saccharomyces cerevisiae has been designed. Using this system, it was shown that: 1—the α-factor pro-peptide has contributed almost equally to the secretion at the Erv29p-dependent stage of vesicular transport and at the translocation stage; 2—of the two above stages, the Erv29p-dependent stage could only be optimized by using of two copies of the α-factor pro-peptide; 3—when the α-factor pre-peptide and the artificial pro-peptide with significantly greater hydrophobicity were compared it was found that somatropin secretion apparently depended on the translocation mode as higher secretion was achieved with hydrophobic artificial pre-peptide associated with the co-translational translocation.
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Abbreviations
- ER:
-
endoplasmic reticulum
- PAGE:
-
polyacrylamide gel electrophoresis
References
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Kazachenko, K.Y., Efremov, B.D. & Kozlov, D.G. Activities of elements of the yeast α-factor precursor leader at different stages of somatropin secretion by Saccharomyces cerevisiae . Appl Biochem Microbiol 50, 829–834 (2014). https://doi.org/10.1134/S000368381409004X
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DOI: https://doi.org/10.1134/S000368381409004X