Applied Biochemistry and Microbiology

, Volume 49, Issue 9, pp 711–722

Expression of eukaryotic recombinant proteins and deriving them from the milk of transgenic animals

  • Yu. M. Khodarovich
  • I. L. Goldman
  • E. R. Sadchikova
  • P. G. Georgiev
Problems and Prospects

DOI: 10.1134/S0003683813090020

Cite this article as:
Khodarovich, Y.M., Goldman, I.L., Sadchikova, E.R. et al. Appl Biochem Microbiol (2013) 49: 711. doi:10.1134/S0003683813090020
  • 195 Downloads

Abstract

Expression in milk of transgenic farm animals is one of the most effective ways to produce eukaryotic recombinant proteins. In this review, we discuss the problems of the appropriateness of selecting this expression system for the production of proteins and the development characteristics of appropriate production processes.

Keywords

biotechnology milk recombinant proteins transgenic animals 

Abbreviations

GEC

genetic engineering construct

G-CSF

granulocyte colony-stimulating factor

monaAB

monoclonal antibodies

RP

recombinant protein

EDTA

ethylenediamine tetraacetic acid

CaP technology

calcium phosphate technology

CHO

Chinese hamster ovary cell line

FDA

Food and Drug Administration (United States)

GAP

Good Agricultural Practice

Copyright information

© Pleiades Publishing, Inc. 2013

Authors and Affiliations

  • Yu. M. Khodarovich
    • 1
  • I. L. Goldman
    • 2
  • E. R. Sadchikova
    • 2
  • P. G. Georgiev
    • 2
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Institute of Gene BiologyRussian Academy of SciencesMoscowRussia