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The composition and structural characteristics of polysaccharides of the brown alga Eisenia bicyclis

  • Algology
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Abstract

The present study examines the polysaccharide composition of the brown alga Eisenia bicyclis. The major polysaccharide of E. bicyclis is shown to be sodium alginate (15.8% of the dry defatted alga weight). The yields of laminaran and fucoidan were 1.4% and 1.3% of the dry defatted alga weight, respectively. The alga contains laminaran (1,3;1,6-β-D-glucan) that has a unique structure, a high content of 1,6-linked D-glucose residues (the ratio of 1,3: 1,6 linkage is 1.5: 1), and an unusually high molecular weight (19–27 kDa). The fucoidan fractions isolated from E. bicyclis are sulfated and acetylated heteropolysaccharides that differ in their sulfate content and monosaccharide composition.

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References

  1. Zvyagintseva, T.N., Elyakova, L.A., and Isakov, V.V., The enzymic transformation of laminarans in 1 → 3;1 → 6-β-D-glucans with immunostimulating activity, Bioorg. Khim., 1995, vol. 21, pp. 218–225.

    CAS  Google Scholar 

  2. Zvyagintseva, T.N., Shirokova, N.I., and Elyakova, L.A., The structure of laminarans from several brown algae, Bioorg. Khim., 1994, vol. 20, pp. 1349–1358.

    CAS  Google Scholar 

  3. Zvyagintseva, T.N., Besednova, N.N., and Elyakova, L.A., Struktura i immunotropnoe deistvie 1,3;1,6-β-D-glyukanov (Structure and Immunotropic Effect of 1,3;1,6-β-D-glucans), Vladivostok: Dal’nauka, 2002.

    Google Scholar 

  4. Usov, A.I., Alginic acids and alginates: methods of analysis and determination of the composition and structure, Usp. Khim., 1999, vol. 68, pp. 1051–1061.

    Article  Google Scholar 

  5. Usov, A.I. and Bilan M.I., Fucoidans-sulphated polysaccharides of brown algae, Usp. Khim., 2009, vol. 78, pp. 846–862.

    Article  Google Scholar 

  6. Bradford, M.M., Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.

    Article  PubMed  CAS  Google Scholar 

  7. Dodgson, K.S. and Price, R.G., A note on the determination of the ester sulphate content of sulphated polysaccharides, Biochem. J., 1962, vol. 84, pp. 106–110.

    PubMed  CAS  Google Scholar 

  8. Dubois, M., Gilles, K.A., Hamilton, J.K., et al., Colorimetric method for determination of sugars and related substances, Anal. Chem., 1956, vol. 28, pp. 350–356.

    Article  CAS  Google Scholar 

  9. Ermakova, S., Men’shova, R., Vishchuk, O., et al., Water-soluble polysaccharides of brown seaweed Eisenia bicyclis. Structural characteristics and antitumor activity, Algal Res., 2013, vol. 2, pp. 51–58.

    Article  Google Scholar 

  10. Grasdalen, H., Larsen B., and Smidsrod, O., A p.m.r. study of the composition and sequence of uronate residues in alginates, Carbohydr. Res., 1979, vol. 68, pp. 23–31.

    Article  CAS  Google Scholar 

  11. Grasdalen, H., Larsen, B., and Smidsrod, O., 13C-N.M.R. studies of monomeric composition and sequence in alginate, Carbohydr. Res., 1981, vol. 89, pp. 179–191.

    Article  CAS  Google Scholar 

  12. Honya, M., Mori, H., Anzai, M., et al., Monthly changes in the content of fucans, their constituent sugars and sulphate in cultured Laminaria japonica, Hydrobiologia, 1999, vol. 398, pp. 411–416.

    Article  Google Scholar 

  13. Kim, Y.T., Kim, E.H., Cheong, C., et al., Structural characterization of β-D-(1 → 3,1 → 6)-linked glucans using NMR spectroscopy, Carbohydr. Res., 2000, vol. 328, pp. 331–341.

    Article  PubMed  CAS  Google Scholar 

  14. Kusaykin, M.I., Bakunina, I.Yu., Sova, V.V., et al., Structure, biological activity, and enzymatic transformation of fucoidans from the brown seaweeds, Biotechnol. J., 2008, vol. 3, pp. 904–915.

    Article  PubMed  CAS  Google Scholar 

  15. Maeda, M. and Nishizawa, K., Fine structure of laminaran of Eisenia bicyclis, J. Biochem., 1968, vol. 63, pp. 199–206.

    PubMed  CAS  Google Scholar 

  16. Pang, Z., Otaka, K., Maoka, T., et al., Structure of β-glucan oligomer from laminarin and its effect of human monocytes to inhibit the proliferation of U937 cells, Biosci. Biotechnol. Biochem., 2005, vol. 69, pp. 553–558.

    Article  PubMed  CAS  Google Scholar 

  17. Singleton, V.L. and Rossi, J.A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Am. J. Enol. Viticult., 1965, vol. 16, pp. 144–158.

    CAS  Google Scholar 

  18. Usui, T., Asari, K., and Mizuno, T., Isolation of highly purified “fucoidan” from Eisenia bicyclis and its anticoagulant and antitumor activities, Agric. Biol. Chem., 1980, vol. 44, pp. 1965–1966.

    Article  CAS  Google Scholar 

  19. Usui, T., Toriyama, T., and Mizuno, T., Structural investigation of laminaran of Eisenia bicyclis, Agric. Biol. Chem., 1979, vol. 43, pp. 603–611.

    Article  CAS  Google Scholar 

  20. Zvyagintseva, T.N., Shevchenko, N.M., Nazarenko, E.L., et al., Water-soluble polysaccharides of some Far-eastern brown seaweeds. Distribution, structure, and their dependence on the developmental conditions, J. Exp. Mar. Biol. Ecol., 2003, vol. 294, pp. 1–13.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Branch, Russian Academy of Sciences, Vladivostok, 690022, Russia

    R. V. Men’shova, S. P. Ermakova & T. N. Zvyagintseva

  2. Korean Institute of Science and Technology, Gangneung Institute, Gangneung, 210-340, Korea

    B. H. Um

Authors
  1. R. V. Men’shova
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  2. S. P. Ermakova
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  3. B. H. Um
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  4. T. N. Zvyagintseva
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Correspondence to R. V. Men’shova.

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Original Russian Text © R.V. Men’shova, S.P. Ermakova, B.H. Um, T.N. Zvyagintseva, 2013, published in Biologiya Morya

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Men’shova, R.V., Ermakova, S.P., Um, B.H. et al. The composition and structural characteristics of polysaccharides of the brown alga Eisenia bicyclis . Russ J Mar Biol 39, 208–213 (2013). https://doi.org/10.1134/S1063074013030103

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  • DOI: https://doi.org/10.1134/S1063074013030103

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