Journal of Analytical Chemistry

, Volume 66, Issue 5, pp 490–495

Titrimetric and spectrophotometric assay of pantoprazole in pharmaceuticals using cerium(IV) sulphate as oxidimetric agent

  • Okram Zenita Devi
  • Kanakapura Basavaiah
  • H. D. Revanasiddappa
  • Kanakapura Basavaiah Vinay
Articles

DOI: 10.1134/S1061934811050133

Cite this article as:
Devi, O.Z., Basavaiah, K., Revanasiddappa, H.D. et al. J Anal Chem (2011) 66: 490. doi:10.1134/S1061934811050133

Abstract

Titrimetric and spectrophotometric assay of pantoprazole sodium sesquihydrate (PSS) using cerium(IV) sulphate as the oxidimetric reagent is described. The methods are based on the oxidation of PSS with a measured excess of Ce(IV) sulphate followed by the determination of unreacted oxidant using different reaction schemes. In titrimetry, the unreacted oxidant was determined by back titration with ferrous ammonium sulphate (FAS) in sulphuric acid medium. Spectrophotometry involves the reduction of unreacted Ce(IV) sulphate with a fixed quantity of Fe(II). The resulting Fe(III) is complexed with thiocyanate and the absorbance is measured at 470 nm. In both the methods, the amount of Ce(lV) sulphate reacted corresponds to PSS concentration. Titrimetry is applicable over 1–10 mg range whereas in spectrophotometry, the calibration graph is linear in the range of 0.5–7.0 μg/mL and the calculated molar absorptivity value is 1.58 × 105 L/mol cm. The validity of the proposed methods was tested by analyzing pure and dosage forms containing PSS. Statistical treatment of the results reflects that the proposed procedures are precise, accurate and easily applicable to the determination of PSS in pure form and in pharmaceutical formulations.

Keywords

pantoprazole sodium sesquihydrate assay spectrophotometry cerimetry pharmaceuticals 

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • Okram Zenita Devi
    • 1
  • Kanakapura Basavaiah
    • 1
  • H. D. Revanasiddappa
    • 1
  • Kanakapura Basavaiah Vinay
    • 1
  1. 1.Department of ChemistryUniversity of Mysore ManasagangotriMysoreIndia