Journal of Analytical Chemistry

, Volume 65, Issue 3, pp 293–297

Development and validation of rapid HPLC method for determination of doxofylline in bulk drug and pharmaceutical dosage forms

  • Ashu Mittal
  • Shikha Parmar
Articles

DOI: 10.1134/S1061934810030147

Cite this article as:
Mittal, A. & Parmar, S. J Anal Chem (2010) 65: 293. doi:10.1134/S1061934810030147

Abstract

A simple, selective, rapid, and economical reversed phase high performance liquid chromatography(RP-HPLC) method for the determination of doxofylline in the commercial dosage form has been developed and validated. The separation and quantification were achieved on an HiQ Sil C 18 W column using a mobile phase of acetonitrile: buffer (50: 50), pH 3, at a flow rate of 1 mL/min with detection of analyte at 272 nm. The separation was achieved within 3.1 ± 0.3 min for doxofylline sample. The method showed good linearity in the range of 10–80 μg/mL. The intra and inter day RSD ranged from 0.37–0.53%. The recovery (mean ± S.D.) of low, middle and high concentrations were 100.04 ± 0.80, 100.01 ± 0.20, 100.07 ± 0.30 respectively. Limit of detection and limit of quantification were 0.03 and 0.1 μg/mL, respectively.

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • Ashu Mittal
    • 1
  • Shikha Parmar
    • 1
  1. 1.Department of PharmaceuticsITS—Paramedical (Pharmacy) CollegeMuradnagar (Ghaziabad)India