Chemical fingerprinting and quantitative analysis of volatiles in Shexiang Baoxin Pill by gas chromatography with flame ionization and mass spectrometric detection
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- Yan, S., Yang, Y., Wu, Y. et al. J Anal Chem (2009) 64: 149. doi:10.1134/S1061934809020105
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An approach to fingerprinting and the quantitative analysis of volatiles in Shexiang Baoxin Pill (SBP) was proposed by using gas chromatography (GC) with a flame ionization detector and a mass spectrometric detector (MS). Using the proposed method, the chemical fingerprint of SBP extract was established, in which the separation of more than 30 volatiles was realized in about 30 min, and 26 peaks were identified by GC/MS analysis. Seven major volatiles, including borneol, isoborneol, isopropyl methylphenol, mascone, cinnamaldehyde, cinnamic acid, and benzyl benzoate, were further quantified. The linearity, precision, stability, repeatability, and accuracy were acceptable. The proposed method was successfully applied to the determination of volatiles in 11 batches of SBP samples. To evaluate their quality, principal components analysis (PCA) was performed on the basis of the data of chemical fingerprints, and the score plot clearly revealed the variations of samples produced in different years. Moreover, the results of the quantitative analysis of various samples were also comparatively studied. The results indicated that, to ensure the quality of SBP, more efficient storage and package techniques are needed. The proposed method enables chemical fingerprinting and the simultaneous determination of multicomponents to be performed in one run, and can be applied as a comprehensive quality control technique for traditional Chinese medicine containing volatile constituents.