Laser Physics

, Volume 20, Issue 7, pp 1673–1678

Study of the efficacy of 5 ALA-mediated photodynamic therapy on human larynx squamous cell carcinoma (Hep2c) cell line

  • A. Khursid
  • M. Atif
  • S. Firdous
  • S. S. Z. Zaidi
  • R. Salman
  • M. Ikram
Laser Methods in Chemistry, Biology, and Medicine

DOI: 10.1134/S1054660X10130086

Cite this article as:
Khursid, A., Atif, M., Firdous, S. et al. Laser Phys. (2010) 20: 1673. doi:10.1134/S1054660X10130086

Abstract

5-aminolevulanic acid (ALA), a precursor of Protoporphyrin IX, was evaluated as an inducer of photodamage on Hep2c, human larynx squamous cell carcinoma, cell line. Porphyrins are used as active cytotoxic antitumor agents in photodynamic therapy (PDT). The present study evaluates the effects of photodynamic therapy (PDT) with 5-aminolevulinic acid (5-ALA) using human larynx cells as experimental model. Hep2c cell line was irradiated with red light (a diode laser, λ = 635 nm). The influence of different incubation times and concentrations of 5-ALA, different irradiation doses and various combinations of photosensitizer and light doses on the cellular viability of Hep2c cells were studied. The optimal uptake of photosensitizer ALA in Hep-2c cells was investigated by means of spectrometric measurement. Cells viability was determined by means of neutral red assay (NR). It was observed that sensitizer or light doses have no significant effect on cells viability when studied independently. The spectrometric measurements showed that the maximal cellular uptake of 5-ALA occurred after 7 h in vitro incubation. The photocytotoxic assay showed that light dose of 85 J/cm2 gives effective PDT outcome for Hep2c cell line incubated with 55 μg/ml of 5-ALA with a conclusion that Hep2c cell line is sensitive to ALA-mediated PDT.

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • A. Khursid
    • 1
  • M. Atif
    • 2
  • S. Firdous
    • 2
  • S. S. Z. Zaidi
    • 3
  • R. Salman
    • 3
  • M. Ikram
    • 1
  1. 1.Pakistan Institute of Engineering and SciencesNilore, IslamabadPakistan
  2. 2.National Institute of Laser and OptronicsNilore, IslamabadPakistan
  3. 3.National Institute of HealthIslamabadPakistan

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