Laser Physics

, Volume 19, Issue 7, pp 1475–1479

In vivo polarization dependant Second and Third harmonic generation imaging of Caenorhabditis elegans pharyngeal muscles

  • G. Filippidis
  • K. Troulinaki
  • C. Fotakis
  • N. Tavernarakis
Laser Methods in Chemistry, Biology, and Medicine

DOI: 10.1134/S1054660X09070184

Cite this article as:
Filippidis, G., Troulinaki, K., Fotakis, C. et al. Laser Phys. (2009) 19: 1475. doi:10.1134/S1054660X09070184

Abstract

In this study Second and Third harmonic generation (SHG-THG) imaging measurements were performed to the pharyngeal muscles of the nematode Caenorhabditis elegans, in vivo with linearly polarized laser beam. Complementary information about the anatomy of the pharynx and the morphology of the anterior part of the worm were extracted. THG signals proved to have no dependence on incident light polarization, while SHG images are highly sensitive to the changes of the incident linearly polarized light.

PACS numbers

87.64.-t87.63.lt87.64.mn87.19.Ff

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • G. Filippidis
    • 1
  • K. Troulinaki
    • 2
  • C. Fotakis
    • 1
    • 3
  • N. Tavernarakis
    • 2
  1. 1.Institute of Electronic Structure and LaserFoundation of Research and Technology-HellasHeraklion, CreteGreece
  2. 2.Institute of Molecular Biology and BiotechnologyFoundation of Research and TechnologyHeraklion, CreteGreece
  3. 3.Department of PhysicsUniversity of CreteCreteGreece