In vivo polarization dependant Second and Third harmonic generation imaging of Caenorhabditis elegans pharyngeal muscles
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- Filippidis, G., Troulinaki, K., Fotakis, C. et al. Laser Phys. (2009) 19: 1475. doi:10.1134/S1054660X09070184
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In this study Second and Third harmonic generation (SHG-THG) imaging measurements were performed to the pharyngeal muscles of the nematode Caenorhabditis elegans, in vivo with linearly polarized laser beam. Complementary information about the anatomy of the pharynx and the morphology of the anterior part of the worm were extracted. THG signals proved to have no dependence on incident light polarization, while SHG images are highly sensitive to the changes of the incident linearly polarized light.