Laser Physics

, Volume 16, Issue 5, pp 884–888

Fluorescence spectroscopy applied to orange trees

  • L. G. Marcassa
  • M. C. G. Gasparoto
  • J. BelasqueJr
  • E. C. Lins
  • F. Dias Nunes
  • V. S. Bagnato
Laser Methods in Biology and Medicine

DOI: 10.1134/S1054660X06050215

Cite this article as:
Marcassa, L.G., Gasparoto, M.C.G., Belasque, J. et al. Laser Phys. (2006) 16: 884. doi:10.1134/S1054660X06050215

Abstract

In this work, we have applied laser-induced fluorescence spectroscopy to investigate biological processes in orange trees (Citrus aurantium L.). We have chosen to investigate water stress and Citrus Canker, which is a disease caused by the Xanthomonas axonopodis pv. citri bacteria. The fluorescence spectroscopy was investigated by using as an excitation source a 442-nm 15-mW HeCd gas multimode discharge laser and a 532-nm 10-mW Nd3+:YAG laser. The stress manifestation was detected by the variation of fluorescence ratios of the leaves at different wavelengths. The fluorescence ratios present a significant variation, showing the possibility to observe water stress by fluorescence spectrum. The Citrus Canker’s contaminated leaves were discriminated from the healthy leaves using a more complex analysis of the fluorescence spectra. However, we were unable to discriminate it from another disease, and new fluorescence experiments are planned for the future.

PACS numbers

07.60.RD07.57.TY82.50.-M

Copyright information

© MAIK “Nauka/Interperiodica” 2006

Authors and Affiliations

  • L. G. Marcassa
    • 1
  • M. C. G. Gasparoto
    • 2
  • J. BelasqueJr
    • 2
  • E. C. Lins
    • 3
  • F. Dias Nunes
    • 3
  • V. S. Bagnato
    • 1
  1. 1.IFSC-Universidade de São PauloSão Carlos, São PauloBrazil
  2. 2.Departmento Cientifico-FUNDECITRUSAraraquara, São PauloBrazil
  3. 3.DES-Universidade Federal de PernambucoRecife, PernambucoBrazil