Russian Journal of Genetics

, Volume 48, Issue 3, pp 336–346

Highly efficient transfer and stable expression of two genes upon lentivirus transduction of mesenchymal stem cells from human bone marrow

  • V. V. Grinev
  • I. N. Seviaryn
  • D. V. Posrednik
  • S. M. Kosmacheva
  • M. P. Potapnev
Human Genetics

DOI: 10.1134/S1022795412030039

Cite this article as:
Grinev, V.V., Seviaryn, I.N., Posrednik, D.V. et al. Russ J Genet (2012) 48: 336. doi:10.1134/S1022795412030039
  • 184 Downloads

Abstract

The efficiency of human bone marrow (BM) mesenchymal stem cell (MSC) transduction with a bicistronic lentivirus vector was estimated, and the stability of transgene expression in genetically modified MSCs was determined. First-passage BM MSCs were capable of efficient transduction with the bicistronic lentivirus vector. The transduction efficiency depended on the multiplicity of infection (MOI), being 64.64 ± 6.5 and 88.6 ± 2.9% at MOI 10 and 20, respectively. The lentivirus transduction efficiency proved independent on the number of passages of a BM MSC culture, and expression of the egfp and dsRed1 transgenes in genetically modified MSCs remained stable for one month of culturing. A comparison showed that the level of egfp and dsRed1 transgene expression was preserved upon hepatogenic differentiation in vitro. The results provide a basis for further development of multigenic modification of human BM MSCs for research and/or therapeutic purposes.

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • V. V. Grinev
    • 1
  • I. N. Seviaryn
    • 2
  • D. V. Posrednik
    • 1
  • S. M. Kosmacheva
    • 2
  • M. P. Potapnev
    • 2
  1. 1.Department of GeneticsBelarussian State UniversityMinskBelarus
  2. 2.Belarussian Research and Production Center for Hematology and TransfusiologyMinskBelarus