Russian Journal of Genetics

, Volume 45, Issue 4, pp 430–439

Molecular cloning and mRNA expression profiling of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Hyoscyamus niger

  • K. Jiang
  • Y. Pi
  • Z. Huang
  • R. Hou
  • Zh. Zhang
  • J. Lin
  • X. Sun
  • K. Tang
Plant Genetics

DOI: 10.1134/S1022795409040073

Cite this article as:
Jiang, K., Pi, Y., Huang, Z. et al. Russ J Genet (2009) 45: 430. doi:10.1134/S1022795409040073

Abstract

In the endeavor to enhance the production of pharmaceutically valuable tropane alkaloids including hyoscyamine and scopolamine in Hyoscyamus niger, methyl jasmonate (MeJA) showed significant stimulation both in tropane biosynthetic pathway enzymes activities and tropane alkaloids yields. Therefore it was speculated that genetic engineering of jasmonate biosynthetic pathway might enhance the endogenous jasmonates concentration, followed by stimulating the production of tropane alkaloids. Herein a full-length cDNA encoding allene oxide synthase (AOS, EC 4.2.1.92), the first committed step enzyme in jasmonate biosynthetic pathway was reported (named HnAOS, GenBank accession: EF532599). HnAOS was a novel member of the cytochrome P450 (CYP74A) subfamily. Real-time quantitative PCR analysis showed that HnAOS mRNA accumulated mainly in stems, and responded significantly to wounding or methyl jasmonate.

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • K. Jiang
    • 1
  • Y. Pi
    • 1
  • Z. Huang
    • 1
  • R. Hou
    • 1
  • Zh. Zhang
    • 1
  • J. Lin
    • 1
  • X. Sun
    • 1
  • K. Tang
    • 1
    • 2
  1. 1.State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Morgan-Tan International Center for Life SciencesFudan UniversityShanghaiChina
  2. 2.Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Institute of Systems BiologyShanghai Jiao Tong UniversityShanghaiChina