Some physiological responses of chickpea cultivars to arbuscular mycorrhiza under drought stress
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
A factorial experiment based on RCB design with three replicates was conducted to investigate changes in some physiological responses of two chickpea (Cicer arietinum L.) cultivars (Pirouz from Desi type and ILC482 from Kabuli type) to arbuscular mycorrhiza (Glomus etunicatum Becker and Gerdman) under different irrigation treatments. The experiment was carried out in the greenhouse of the Agricultural Faculty of Kurdistan University from April to August 2009. The results showed that leaf chlorophyll content of chickpea cultivars was significantly increased by arbuscular mycorrhiza (AM) under both well and limited irrigation conditions. Proline accumulation in chickpea leaves under moderate and severe drought stresses was significantly stronger than that under optimum irrigation. Inoculation of chickpea with mycorrhizal fungi caused an increase in the activities of polyphenol oxidase and peroxidase, but a decrease in the activity of catalase. Comparisons among different irrigation levels showed that chickpea plants under drought stress had the most active lipid peroxidation. Non-AM plants showed stronger lipid peroxidation under moderate and severe water stresses than AM plants. Lipid peroxidation was more active in Pirouz leaves than in ILC482 leaves. It seems that Kabuli-type cultivar responded better to mycorrhizal symbiosis under drought stress than Desitype cultivar.
- Al-Karaki, G.N., Benefit, Cost and Water-Use Efficiency of Arbuscular Mycorrhizal Durum Wheat Grown under Drought Stress, Mycorrhiza, 1998, vol. 8, pp. 41–45. CrossRef
- Beltrano, J., Montaldi, E.R., Bartoli, C.G., and Carbone, A., Emission of Water Stress Ethylene in Wheat (Triticum aestivum L.) Ears: Effects of Rewatering, Plant Growth Regul., 1997, vol. 21, pp. 121–126. CrossRef
- Dionisio-Sese, M.L. and Tobita, S., Antioxidant Responses of Rice Seedlings to Salinity Stress, Plant Sci., 1998, vol. 135, pp. 1–9. CrossRef
- Scandalios, J.G., Oxygen Stress and Superoxide Dismutases, Plant Physiol., 1993, vol. 101, pp. 7–12.
- Burke, J.J. and Mahan, J.R., Environmental Regulation of Cellular Protection Systems, Plant Biochemical Regulators, Gausman, H.W., Ed., New York: Marcel Dekker, 1991, pp. 47–58.
- Girousse, C., Bournoville, R., and Bonnemain, J.L., Water Deficit-Induced Changes in Concentrations in Proline and Some Other Amino Acids in the Phloem Sap of Alfalfa, Plant Physiol., 1996, vol. 111, pp. 109–113.
- Gadkar, V., David-Schwartz, R., Kunik, T., and Kapulnik, Y., Arbuscular Mycorrhizal Fungal Colonization. Factors Involved in Host Recognition, Plant Physiol., 2001, vol. 127, pp. 1493–1499. CrossRef
- Porcel, R., Barea, J.M., and Ruiz-Lozano, J.M., Antioxidant Activities in Mycorrhizal Soybean Plants under Drought Stress and Their Possible Relationship to the Process of Nodule Senescence, New Phytol., 2003, vol. 157, pp. 135–143. CrossRef
- Lambais, M.R., Rios-Ruiz, W.E., and Andrade, R.M., Antioxidant Responses in Bean (Phaseolus vulgaris) Roots Colonized by Arbuscular Mycorrhizal Fungi, New Phytol., 2003, vol. 160, pp. 421–428. CrossRef
- Robinson, D.A., Campbell, C.S., Hopmans, J.W., Hornbuckle, B.K., Jones, S.B., Knight, R., Ogden, F., Selker, J., and Wendroth, O., Soil Moisture Measurement for Ecological and Hydrological Watershed-Scale Observatories: A Review, Vadose Zone J., 2008, vol. 7, pp. 358–389. CrossRef
- Harborne, J.B., Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis, London: Chapman and Hall, 1984.
- Lichtenthaler, H.K. and Wellburn, A.R., Determinations of Total Carotenoids and Chlorophylls a and b of Leaf Extracts in Different Solvents, Biochem. Soc. Trans., 1983, vol. 11, pp. 591–592.
- Bradford, M.N., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248–254. CrossRef
- Bates, L.S., Waldran, R.P., and Teare, I.D., Rapid Determination of Free Proline for Water Studies, Plant Soil, 1973, vol. 39, pp. 205–208. CrossRef
- Beers, R.F., Jr. and Sizer, I.W., A Spectrophotometric Method for Measuring the Breakdown of Hydrogen Peroxide by Catalase, J. Biol. Chem., 1952, vol. 195, pp. 133–140.
- Hemeda, H.M. and Klein, B.P., Effects of Naturally Occurring Antioxidants on Peroxidase Activity of Vegetable Extracts, J. Food Sci., 1990, vol. 55, pp. 184–185. CrossRef
- Kumar, K.B. and Khan, P.A., Peroxidase and Polyphenoloxidase in Excised Ragi (Eleusine coracana cv. PR 202) Leaves during Senescence, Ind. J. Exp. Bot., 1982, vol. 20, pp. 412–416.
- Nakano, Y. and Asada, K., Hydrogen Peroxide Is Scavenged by Ascorbate Specific Peroxidase in Spinach Chloroplasts, Plant Cell Physiol., 1981, vol. 22, pp. 867–880.
- Cakmak, I. and Horst, J., Effect of Aluminium on Lipid Peroxidation, Superoxide Dismutase, Catalase, and Peroxidase Activities in Root Tips of Soybean (Glycine max), Physiol. Plant., 1991, vol. 83, pp. 463–468. CrossRef
- Sánchez-Blanco, M.J., Fernández, T., Morales, M.A., Morte, A., and Alarcón, J.J., Variations in Water Status, Gas Exchange, and Growth in Rosmarinus officinalis Plants Infected with Glomus deserticola under Drought Conditions, J. Plant Physiol., 2004, vol. 161, pp. 675–682. CrossRef
- Wu, Q.S., Zou, Y.N., and Xia, R.X., Effects of Water Stress and Arbuscular Mycorrhizal Fungi on Reactive Oxygen Metabolism and Antioxidant Production by Citrus (Citrus tangerine) Roots, Eur. J. Soil Biol., 2006, vol. 42, pp. 166–172. CrossRef
- Alexieva, V., Sergiev, I., Mapelli, S., and Karanov, E., The Effect of Drought and Ultraviolet Radiation on Growth and Stress Markers in Pea and Wheat, Plant Cell Environ., 2001, vol. 24, pp. 1337–1344. CrossRef
- Foyer, C.H., Descourvieres, P., and Kunert, K.J., Protection against Oxygen Radicals: An Importance Defence Mechanism Studied in Transgenic Plants, Plant Cell Environ., 1994, vol. 76, pp. 507–523. CrossRef
- Bowler, C., van Camp, W., van Montagu, M., and Inze, D., Superoxide Dismutase in Plants, Crit. Rev. Plant Sci., 1994, vol. 13, pp. 199–218.
- He, Z., He, C., Zhang, Z., Zou, Z., and Wang, H., Changes of Antioxidative Enzymes and Cell Membrane Osmosis in Tomato Colonized by Arbuscular Mycorrhiza under NaCl Stress, Colloids and Surfaces, B: Biointerfaces, 2007, vol. 59, pp. 128–133. CrossRef
- Halliwell, B. and Gutteridge, J.M.C., Free Radicals in Biology and Medicine, Oxford (UK): Claredon Press, 1989.
- Some physiological responses of chickpea cultivars to arbuscular mycorrhiza under drought stress
Russian Journal of Plant Physiology
Volume 59, Issue 6 , pp 708-716
- Cover Date
- Print ISSN
- Online ISSN
- SP MAIK Nauka/Interperiodica
- Additional Links
- Cicer arietinum
- arbuscular mycorrhiza
- chlorophyll content
- drought stress
- Author Affiliations
- 1. Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan University, Sanandaj, Iran
- 2. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Tabriz University, Tabriz, Iran
- 3. Department of Agronomy, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran