Specific features of oxidative stress in the chilled tobacco plants following transformation with the desC gene for acyl-lipid Δ9-desaturase from Synechococcus vulcanus
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Tobacco plants (Nicotiana tabacum L.) transformed with the desC gene for acyl-lipid Δ9-desaturase from a thermophilic cyanobacterium Synechococcus vulcanus were cultivated on the agarized Murashige and Skoog medium at 22°C and a 16-h photoperiod. Tobacco plants transformed with an empty binary vector pGA482 served as the control. The investigations showed that, in contrast to the control, transgenic plants maintained a higher activity of antioxidant enzymes during 2-h incubation at 2°C; as a result, these plants resisted more efficiently the accumulation of reactive oxygen species and reduced the rate of the lipid peroxidation. The activity of antioxidant enzymes in the transformed plants is apparently related to the operation of the introduced desC gene for acyl-lipid Δ9-desaturase because the enhanced activity of the latter increased the relative content of polyunsaturated FAs in membrane lipids and in this way promoted the liquid state of membranes during the chilling period. These changes helped preserve the cellular homeostasis and thereby maintain the steady synthesis of antioxidant enzymes at hypothermic conditions; as a result, cold resistance of transformed tobacco plants increased.
- Hariadi, P. and Parkin, K.L., Chilling Induced Oxidative Stress in Cucumber Seedling, J. Plant Physiol., 1993, vol. 141, pp. 733–738.
- Prasad, T.K., Anderson, M.D., Martin, B.A., and Stewart, C.R., Evidence for Chilling-Induced Oxidative Stress in Maize Seedlings and a Regulatory Role for Hydrogen Peroxide, Plant Cell, 1994, vol. 6, pp. 65–74. CrossRef
- Lukatkin, A.S., Kholodovoe povrezhdenie teplolyubivykh rastenii i okislitel’nyi stress (Cold Damage to Chilling-Sensitive Plants and Oxidative Stress), Saransk: Mordov. Gos. Univ., 2002.
- Merzlyak, M.N., Aktivirovannyi kislorod i okislitel’nye protsessy v membranakh rastitel’noi kletki (Activated Oxygen and Oxidative Processes in Plant Cell Membranes), Itogi Nauki i Tekhniki, Ser. Fiziol. Rast., 1989, vol. 6.
- Vladimirov, Yu.A. and Archakov, A.P., Perekisnoe okislenie lipidov biologicheskikh membran (Lipid Peroxidation in Biological Membranes), Moscow: Nauka, 1972.
- Mazliak, P., Glyco-and Phospholipids of Biomembranes in Higher Plants, Lipids and Lipid Polymers in Higher Plants, Berlin: Springer-Verlag, 1977, pp. 48–77.
- Baraboi, V.A., Mechanisms of Stress and Lipid Peroxidation, Usp. Sovrem. Biol., 1991, vol. 111, pp. 923–931.
- Orlova, I.V., Serebriiskaya, T.S., Popov, V.N., Merkulova, N.V., Nosov, A.M., Trunova, T.I., Tsydendambaev, V.D., and Los, D.A., Transformation of Tobacco with a Gene for the Thermophilic Acyl-Lipid Desaturase Enhances the Chilling Tolerance of Plants, Plant Cell Physiol., 2003, vol. 44, pp. 447–450. CrossRef
- Popov, V.N., Orlova, I.V., Kipaikina, N.V., Serebriiskaya, T.S., Merkulova, N.V., Nosov, A.M., Trunova, T.I., Tsydendambaev, V.D., and Los, D.A., The Effect of Tobacco Plant Transformation with a Gene for Acyl-Lipid Δ9-Desaturase from Synechococcus vulcanus on Plant Chilling Tolerance, Fiziol. Rast. (Moscow), 2005, vol. 52, pp. 747–750 (Russ. J. Plant Physiol., Engl. Transl., pp. 664–667).
- Brennan, T. and Frenkel, C., Involvement of Hydrogen Peroxide in the Regulation of Senescence in Pear, Plant Physiol., 1977, vol. 59, pp. 411–416. CrossRef
- Zhirov, V.K., Merzlyak, M.N., and Kuznetsov, L.V., Lipid Peroxidation in Membranes from Cold-Resistant Plants under Below-Zero Temperature Damage, Fiziol. Rast. (Moscow), 1982, vol. 29, pp. 1045–1052 (Sov. Plant Physiol., Engl. Transl.).
- Beauchamp, Ch. and Fridovich, I., Superoxide Dismutase Improved Assays and an Assay Applicapable to Acrylamide Gels, Anal. Biochem., 1971, no. 3, pp. 276–287.
- Kumar, C.N. and Knowles, N., Changes in Lipid Peroxidation and Lipolytic and Free-Radical Scavenging Enzyme during Aging and Sprouting of Potato (Solanum tuberosum L.) Seed-Tubers, Plant Physiol., 1993, vol. 102, pp. 115–124.
- Bradford, M.M., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248–254. CrossRef
- Dospekhov, B.A., Metodika opytnogo dela (Methods for Experiments), Moscow: Kolos, 1977.
- Chen, Yi-Zhu and Patterson, B.D., The Effect of Chilling Temperature on the Level of Superoxide Dismutase, Catalase and Hydrogen Peroxide in Some Plant Leaves, Acta Phytophysiol. Sin., 1988, vol. 14, pp. 323–328.
- Gianinetti, A., Cantoni, M., Lorenzoni, C., Salamini, F., and Marocco, A., Altered Levels of Antioxidant Enzymes Associated with Two Mutations in Tomato, Physiol. Plant., 1993, vol. 89, pp. 157–164. CrossRef
- Okane, D., Gill, V., Boyd, P., and Burdon, B., Chilling, Oxidative Stress and Antioxidant Responses in Arabidopsis thaliana Callus, Planta, 1996, vol. 198, pp. 371–377. CrossRef
- Zhang, J.X., Cui, S.P., Li, J.M., Wei, J.K., and Kirkham, M.B., Protoplasmic Factors, Antioxidant Responses, and Chilling Resistance in Maize, Plant Physiol. Biochem., 1995, vol. 33, pp. 567–575.
- Specific features of oxidative stress in the chilled tobacco plants following transformation with the desC gene for acyl-lipid Δ9-desaturase from Synechococcus vulcanus
Russian Journal of Plant Physiology
Volume 53, Issue 4 , pp 469-473
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Nicotiana tabacum
- transgenic plants
- acyl-lipid desaturase
- antioxidant enzymes
- cold resistance
- Industry Sectors