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Involvement of serine/threonine protein kinases in the cold stress response in the cyanobacterium Synechocystis sp. PCC 6803: Functional characterization of SpkE protein kinase

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Abstract

Stress responses of the unicellular cyanobacterium Synechocystis involve several regulatory systems, including two-component ones, and negative supercoiling of genomic DNA. The role of serine/threonine protein kinases (STPKs) in the cold response was studied in Synechocystis. A screening of a collection of STPK mutants identified four enzymes—SpkB, SpkD, SpkE, and SpkG—as possible transcriptional regulators at lower temperatures. A proteome analysis in a SpkE Synechocystis mutant implicated SpkE in the formation of the protein pattern. In vitro phosphorylation assays of recombinant SpkE confirmed that the STPK was functionally active and utilized basic proteins as preferable substrates.

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia

    A. A. Zorina, V. S. Bedbenov, G. V. Novikova, V. B. Panichkin & D. A. Los’

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  1. A. A. Zorina
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  2. V. S. Bedbenov
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  3. G. V. Novikova
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  4. V. B. Panichkin
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  5. D. A. Los’
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Correspondence to A. A. Zorina.

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Original Russian Text © A.A. Zorina, V.S. Bedbenov, G.V. Novikova, V.B. Panichkin, D.A. Los’, 2014, published in Molekulyarnaya Biologiya, 2014, Vol. 48, No. 3, pp. 452–462.

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Zorina, A.A., Bedbenov, V.S., Novikova, G.V. et al. Involvement of serine/threonine protein kinases in the cold stress response in the cyanobacterium Synechocystis sp. PCC 6803: Functional characterization of SpkE protein kinase. Mol Biol 48, 390–398 (2014). https://doi.org/10.1134/S0026893314030212

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  • DOI: https://doi.org/10.1134/S0026893314030212

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