Molecular Biology

, Volume 48, Issue 3, pp 305–318

TALE nucleases as a new tool for genome editing


DOI: 10.1134/S0026893314030054

Cite this article as:
Glazkova, D.V. & Shipulin, G.A. Mol Biol (2014) 48: 305. doi:10.1134/S0026893314030054


Introducing targeted changes in the genome of living cells or whole organisms makes it possible to solve many problems of basic science, biotechnology, and medicine. Target gene knockout in zygotes helps to study the functions of the gene in the corresponding organisms, while replacement of single nucleotide in DNA provides an opportunity to correct gene mutations and to treat hereditary disorders. Adding a gene into a proper genome region can be used to construct producer cells or organisms with certain properties. Such genomic manipulations are possible due to the technology known as genome editing. In this technology, a break is introduced into a certain chromosomal DNA region with an endonuclease recognizing a unique sequence, and DNA integrity is then restored by cell repair systems. Custom-designed endonucleases able to cleave a selected target sequence are necessary tools for genome editing. Programmable endonucleases of a new type were constructed on the basis of bacterial transcription activator-like (TAL) effectors (TALEs), marking an important step in the development of genome editing and promoting its broad application. The review considers the history of discovering TALEs and creating TALE nucleases and describes their advantages over zinc finger endonucleases, which were constructed earlier. A section focuses on the genetic modifications that can be performed using various genome editing techniques.


genome editingTALE nucleasezinc finger nucleasehomologous recombinationnonhomologous end joining



transcription activator-like effector

ZF nuclease

zinc finger nuclease

Copyright information

© Pleiades Publishing, Inc. 2014

Authors and Affiliations

  1. 1.Central Research Institute of EpidemiologyFederal Service for Consumer Rights Protection and Human WelfareMoscowRussia